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- 文献和实验
- 技术资料
- 英文名:
Recombinant Cynomolgus B7-H7/HHLA2 Protein,His Tag
- 保存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
- 规格:
25 μg(95451ES25)/100 μg(95451ES60)/500 μg(95451ES76)
| 规格: | 25 μg(95451ES25) | 产品价格: | ¥1195 |
|---|---|---|---|
| 规格: | 100 μg(95451ES60) | 产品价格: | ¥3585 |
| 规格: | 500 μg(95451ES76) | 产品价格: | ¥12355 |
产品属性:
| 产品别称 | B₇H₇, B7-H7, HHLA2, B7 Homolog 7 |
| 种属 | Cynomolgus |
| 表达序列 | Ile21-Asn345 |
| Accession | XP_005548285.2 |
| 标签 | C-His |
| 表达系统 | HEK293 |
| 分子量 | The protein has a predicted MW of 38.31 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE and HPLC. |
| 内毒素 | < 1.0 EU per μg by the LAL method. |
| 制剂 | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 外观 | Lyophilized Powder |
| 生物活性 | 【SPR Data】Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay . |
| 复溶方法 | 开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。 |
产品特色:
高纯度、高活性、低内毒素、高批间一致性
储存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
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文献和实验One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
For recombinant expression of extracellular matrix (ECM) proteins or their individual domains, the use of transformed mammalian cells offers two major advantages. First, eukaryotic expression can be expected under optimum conditions
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