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- 文献和实验
- 技术资料
- 英文名:
Recombinant Human OSMR-GPL fusion Protein,His Tag
- 保存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
- 规格:
25 μg(95273ES25)/100 μg(95273ES60)/500 μg(95273ES76)
| 规格: | 25 μg(95273ES25) | 产品价格: | ¥1455 |
|---|---|---|---|
| 规格: | 100 μg(95273ES60) | 产品价格: | ¥5295 |
| 规格: | 500 μg(95273ES76) | 产品价格: | ¥17685 |
产品属性:
| 产品别称 | OSMR-GPL |
| 种属 | Human |
| 表达序列 | Met1-Ala428 (OSMR) and Leu21-Glu225 (GPL) |
| Accession | Q99650-1(OSMR)&Q₈NI₁₇-1(GPL) |
| 标签 | C-His |
| 表达系统 | HEK293 |
| 分子量 | The protein has a predicted MW of 75.84 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on SDS-PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE. |
| 内毒素 | < 1.0 EU per μg by the LAL method. |
| 制剂 | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 外观 | Lyophilized Powder |
| 生物活性 | 【ELISA Data】 Immobilized Human OSMR-GPL fusion Protein, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. |
| 复溶方法 | 开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。 |
产品特色:
高纯度、高活性、低内毒素、高批间一致性
储存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
(9 ). Because its systemic administration was shown to mediate the regression of some mouse tumors (10 ), TNF-α has attracted much attention as a potential antitumor reagent (11 ). However, the problem of its dose-dependent toxicity has been particularly apparent in human
solubilizing agents, having frequently been observed to improve the yield, enhance the solubility, and promote the proper folding of its fusion partners. The use of a dual hexahistidine–maltose-binding protein affinity tag (His6 –MBP) has the additional
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