- ￥1195 - 12355
- 翌圣生物
- 95064ES
- 2026年10月15日
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- 文献和实验
- 技术资料
- 英文名：
Recombinant Human APOE3/Apolipoprotein E Protein,hFc Tag
- 保存条件：
-25 ~ -15℃保存，收到货之后有效期1年。 复溶后， 无菌条件下，-85 ~ -65℃保存，3个月有效期。
- 规格：
25 μg(95064ES25)/100 μg(95064ES60)/500 μg(95064ES76)
| 规格： | 25 μg(95064ES25) | 产品价格： | ￥1195 |
|---|---|---|---|
| 规格： | 100 μg(95064ES60) | 产品价格： | ￥3585 |
| 规格： | 500 μg(95064ES76) | 产品价格： | ￥12355 |
产品属性：
| 产品别称 | APOE,APOE3,Apo-E,Apolipoprotein E |
| 种属 | Human |
| 表达序列 | Lys19-His317 |
| Accession | P02649 |
| 标签 | N-hFc |
| 表达系统 | HEK293 |
| 分子量 | The protein has a predicted MW of 59.76 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE. |
| 内毒素 | < 1.0 EU per μg by the LAL method. |
| 制剂 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 外观 | Lyophilized Powder |
| 生物活性 | 【SPR Data】Human TREM2, His Tag captured on CM5 Chip via anti-his antibody can bind Human APOE3, hFc Tag with an affinity constant of 0.41 μM as determined in SPR assay . |
| 复溶方法 | 开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 |
产品特色：
高纯度、高活性、低内毒素、高批间一致性
储存条件：
-25 ~ -15℃保存，收到货之后有效期1年。 复溶后， 无菌条件下，-85 ~ -65℃保存，3个月有效期。
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文献和实验Medium-Throughput Production of Recombinant Human Proteins: Protein Production in E. coli
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E . coli system for test expressing intracellular
Resolving Bottlenecks for Recombinant Protein Expression in E. coli
regarding expression of recombinant proteins in E. coli . However, despite this progress, protein production, primarily of eukaryotic origin, still remains a challenge. The biggest obstacle lies in obtaining large amounts of a given protein in a correctly
Apolipoprotein E Expression and Purification
Since the discovery of the association of apolipoprotein E (apoE) 4 with Alzheimer’s disease 17 years ago, numerous in vitro experiments with the apoE isoforms (apoE2, apoE3, and apoE4) have been performed to try to understand the basis
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