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- 文献和实验
- 技术资料
- 英文名:
Recombinant Mouse Alkaline Phosphatase Protein (Intestinal type) ,His Tag
- 保存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
- 规格:
100 μg(94644ES60)/500 μg(94644ES76)/25 μg(94644ES25)
| 规格: | 100 μg(94644ES60) | 产品价格: | ¥4445 |
|---|---|---|---|
| 规格: | 500 μg(94644ES76) | 产品价格: | ¥14855 |
| 规格: | 25 μg(94644ES25) | 产品价格: | ¥1215 |
产品属性:
| 产品别称 | Akp2,IAP, ALPI |
| 种属 | Mouse |
| 表达序列 | Ile21-Gly501 |
| Accession | NP_001074551.1 |
| 标签 | C-His |
| 表达系统 | HEK293 |
| 分子量 | The protein has a predicted MW of 53.10 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE. |
| 内毒素 | < 1.0 EU per μg by the LAL method. |
| 制剂 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 外观 | Lyophilized Powder |
| 复溶方法 | 开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。 |
产品特色:
高纯度、高活性、低内毒素、高批间一致性
储存条件:
-25 ~ -15℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
5 above. Fixation for > 5 minutes can lead to decreased enzyme activity. STAINING FOR ALKALINE PHOSPHATASE ACTIVITY Phosphatase genes are useful reporter genes as several histochemical methods that yield precipitated, highly colored and/or electron
Secondary antibody: Goat anti-mouse alkaline phosphatase conjugated - Biorad #170-6520. Substrate: ECF chemifluorescent substrate - Amersham #RPN5785. Mix substrate with accompanying buffer as per manufacturer’s
Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers
A large-scale stirred culture system for the expansion of mouse embryonic stem cells (mESCs) in spinner flasks under serum-free conditions was established using macroporous microcarriers for cell attachment and growth. This type
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