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- 文献和实验
- 技术资料
- 英文名:
Recombinant Human EDNRB Protein,Flag-His tag (Detergent, Full length)
- 保存条件:
-85 ~ -65℃保存,收到货之后有效期6个月。避免反复冻融,打开小瓶前先离心。建议等分分装为一次实验使用量,分装后,-80℃冻存。
- 规格:
100 μg(94506ES60)/25 μg(94506ES25)
| 规格: | 100 μg(94506ES60) | 产品价格: | ¥24715 |
|---|---|---|---|
| 规格: | 25 μg(94506ES25) | 产品价格: | ¥9885 |
产品描述:
内皮素受体B(EDNRB)是一种G蛋白偶联受体,其活性和功能由激活磷脂酰肌醇钙第二信使系统的G蛋白调节。EDNRB膜蛋白广泛存在于人体内,参与许多生理活动的调节,如与ET结合,并通过细胞内Ca2+磷脂依赖性蛋白激酶第二信使系统将信息传递到细胞内,促进内质网和肌浆网中钙的快速释放。内皮素通过其受体(ETR)(如EDNRB)发挥其生物学功能。内皮素和ETR构成内皮素轴。内皮素轴在各种肿瘤中具有促肿瘤作用,可以促进肿瘤生长和转移,促进抗凋亡和血管生成。
产品属性:
| 产品别称 | Endothelin receptor type B, EDNRB, ABCDS, HSCR, HSCR2, ET-B, ET-BR, ETB1, ETBR, ETRB, WS4A |
| 种属 | Human |
| 表达序列 | Wild type full length |
| Accession | O14493 |
| 标签 | N-His-Flag |
| 表达系统 | Baculovirus-Insect Cells |
| 分子量 | 47 kDa |
| 纯度 | > 90% as determined by SDS-PAGE and HPLC. |
| 内毒素 | < 0.1 EU per µg by the LAL method. |
| 制剂 | 10 mM Hepes, pH 7.5, 200 mM NaCl, 0.01 % LMNG, 0.001 % CHS. |
| 外观 | Liquid |
| 生物活性 | 【ELISA Data】 Immobilized EDNRB Rabbit pAb (A2908) at 0.5μg/mL (100μL/well) can bind EDNRB protein Flag-His tag (detergent, full length), Human (Cat. No. UA060002) with EC50 of 35.50-76.59ng/mL |
产品特色:
高纯度、高活性、低内毒素、高批间一致性
应用案例:
ELISA Data
Figure 1.Immobilized EDNRB Rabbit pAb at 0.5μg/mL (100μL/well) can bind Human
EDNRB protein Flag-His tag with EC50 of 35.50-76.59ng/mL.
储存条件:
-85 ~ -65℃保存,收到货之后有效期6个月。
避免反复冻融,打开小瓶前先离心。建议等分分装为一次实验使用量,分装后,-80℃冻存。
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文献和实验Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer
blotting and employed this method to investigate cleavages generated by recombinant human Dicer in the synthetic miRNA precursors. We paid special care to visualize clearly the cleavages generated by the individual RNase III domains of Dicer
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
a methodological workflow based on an SF?TAP tag comprising a doublet Strep?tag II and a FLAG moiety optimized for rapid as well as efficient tandem affinity purification of native proteins and protein complexes in higher eukaryotic cells. Depending
Construction of Recombinant Human Cytomegalovirus
genome. These studies can extend to the introduction of viral or foreign genetic material into ectopic sites in the viral genome, to investigate viral cis -control sequences or to phenotypically modify or tag the recombinant virus. Genetically modified
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