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- 文献和实验
- 技术资料
- 英文名:
Biotinylated Rhesus macaque CD5 Protein, His-Avi Tag
- 保存条件:
-85 ~ -65℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
- 规格:
100 μg(93172ES60)/500 μg(93172ES76)/1 mg(93172ES80)/25 μg(93172ES25)
| 规格: | 100 μg(93172ES60) | 产品价格: | ¥6885 |
|---|---|---|---|
| 规格: | 500 μg(93172ES76) | 产品价格: | ¥24115 |
| 规格: | 1 mg(93172ES80) | 产品价格: | ¥43865 |
| 规格: | 25 μg(93172ES25) | 产品价格: | ¥2285 |
产品描述:
CD5, also known as Leu-1, Ly-1, and T1, is a 67 kDa transmembrane glycoprotein in the scavenger receptor superfamily. CD5 has been shown to interact homophilically, with CD72 on B cells, and with beta-glucan components of fungal cell walls. CD5 expression on developing thymocytes is positively regulated by signaling through the T cell antigen receptor (TCR) and is upregulated on tolerized peripheral CD4+ cells. It inhibits TCR signaling and promotes T cell nonresponsiveness and survival. CD5 signaling inhibits the generation of regulatory T cells but promotes the development of Th17 cells. Within the B cell lineage, CD5 is expressed on B-1a cells, anergic B cells, and IL-10 producing regulatory B cells. CD5 is also involved in the cellular entry of hepatitis C virus into T cells.
产品属性:
| 产品别称 | CD5 molecule, CD5, LEU1, T1, CD5 antigen |
| 种属 | Rhesus macaque |
| 表达序列 | Arg25-Pro375 |
| Accession | F₆V₅Q₉ |
| 标签 | C-His-Avi |
| 表达系统 | HEK293 |
| 分子量 | The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result. |
| 纯度 | > 95% as determined by SDS-PAGE and HPLC. |
| 内毒素 | < 1.0 EU per μg by the LAL method. |
| 制剂 | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 外观 | Lyophilized Powder |
| 生物活性 | 【ELISA Data】Immobilized Biotinylated Rhesus macaque CD5, His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-CD5 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. |
| 复溶方法 | 开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。 |
产品特色:
高纯度、高活性、低内毒素、高批间一致性
应用案例:
储存条件:
-85 ~ -65℃保存,收到货之后有效期1年。 复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
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文献和实验Application of siRNA Against SARS in the Rhesus Macaque Model
) inhibitors. A pair of siRNA duplexes identified as potent SCV inhibitors in vitro was evaluated for in vivo efficacy and safety in a rhesus macaque SARS model using intranasal administration with clinical viable delivery carrier in three dosing regimens
HABA/Avidin”。 3. 吸取100 ul 生物素标记的蛋白于杯中并测定500 nm 的吸光度值,记为A500 HABA/Avidin/Biotin (数值必须恒定15s 以上)如果A500 HABA/Avidin/Biotin的值不超过(≤)0.3,重新稀释待测样品并检测。 注:计算结果时必须考虑稀释度。 4. 计算Biotin/Protein摩尔比 ①A=生物素化的蛋白毫摩尔浓度=蛋白浓度(mg/ml)/蛋白的分子量 ②B=500nm处的吸光度变化 △
CD5 分子 CD5 常用单克隆抗体或代号:T1,UCHT2,T101,Leul 主要表达细胞:T,Thy,BsubC T细胞 分子质量(kDa)和结构:gp67(清除剂受体) 功 能:与CD72结合,T细胞信号转导和增殖,CD5+ B细胞与自身免疫有关 CD5 Belongs to ancient scavenger receptor family
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