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细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海臻诺生物科技有限公司
Roche 03246779001(3246779001)MagNA Pure LC Total Nucleic Acid Isolation Kit - Lysis/Binding Buffer Refill
MagNA Pure LC2.0 总核酸提取试剂盒配套裂解 / 结合缓冲液补充装,单独补货用,不包含磁珠、蛋白酶 K、洗涤液等其他组分,适配 Total Nucleic Acid Isolation Kit(03264793001)
✅ 基础规格
- 货号:03246779001
- 全称:MagNA Pure LC Total Nucleic Acid Isolation Kit - Lysis/Binding Buffer Refill
- 用途:裂解样本、灭活 RNase/DNase,高盐环境下使核酸结合至磁珠
- 适配仪器:MagNA Pure LC 2.0
❗仅配套 MagNA Pure LC 总核酸提取方案,不可单独完成完整提取,其余试剂仍需主试剂盒或其他补充装
🧪核心特点
- 含高浓度异硫氰酸胍的裂解结合缓冲液,一步实现细胞裂解、核酸酶灭活、DNA+RNA 同时结合磁珠
- 适合血浆、血清、全血、细胞、拭子样本,维持病毒 RNA 稳定性,降低核酸降解
- 补充装设计,实验室主试剂盒其他组分尚有剩余、仅裂解结合液耗尽时单独采购,节约成本
- 提取产物直接用于 RT-qPCR、LightMix 病毒 / 突变检测
🧬应用场景
- MagNA Pure LC2.0 自动化提取总核酸(DNA+RNA)配套耗材补货
- 低拷贝病毒样本前处理(H5N1、H7N9、H1N1 sw 等流感样本)
搭配主试剂盒:03264793001 MagNA Pure LC Total NA Large Volume
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文献和实验Isolation of RNA from Difficult Tissues
, and offer troubleshooting techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion's
Isolation of Cell-Free DNA from Maternal Plasma Using Manual and Automated Systems
system using the MagNA Pure LC instrument (Roche Diagnostics). Use of the methods described here will help to ensure maximum yield and purity of cell-free fetal DNA extracted from maternal plasma samples for downstream analyses.
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
. For Total Nucleic acid isolation, proceed step 8-12. For RNA and DNA isolation, proceed step 13-25. Total Nucleic acid Isolation: 8. Add equal volume (400 μL) of isopropanol. Mix throughly by vortexing for 30 seconds. Incubate the tube at room
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