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- 文献和实验
- 技术资料
- 供应商:
合肥瑞辕生物科技有限公司
- 保修期:
Replacement Glass Shaft, 7mm, 1000mL Standard Spinner Flask
- 规格:
EA
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文献和实验GLASS BEADS 0.45 - 0.5mm beads (Sigma) Clean by soaking in nitric acid -->stir bar, O/N.** **Note, dispose of acid in appropriate flask. Wash w/ lots distilled water
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
stock first 100X in media, then 10x to protoplasts in media. Phytohormones ABA: [active] 50mM, stock 50mM, MW 264, stock = Sigma A1012 13.2mg/ml EtOH, dilute 1000x GA: [active] 10mM, stock 10mM, MW 346, stock = Sigma G7645 34.6mg/ml EtOH
BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
a single colony from a freshly streaked selective plate, and inoculate a starter culture of 2-5 ml LB or YT medium containing the appropriate selective antibiotic. Incubate for ~ 20-24 hr at 37°C with vigorous shaking (~ 300 rpm). Use a flask
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