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- 文献和实验
- 技术资料
- 供应商：
合肥瑞辕生物科技有限公司
- 保修期：
BioResolve SEC mAb Guard, 200 Å, 2.5 µm, 4.6 x 30 mm, 1/pk
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
. For cytospin slides : i) fix air dried cell samples with a freshly prepared paraformaldeyde solution (4% in PBS, pH 7.4) for 30 min at room temperature; ii) rinse slides with PBS and incubate in permeabilisation solution (0.1% Triton X-100, 0.1% sodium citrate
In Situ Cell Death (Apoptosis) Detection by TUNEL labeling
. Water rinse until clear 70% EtOH, 30 sec. 95% EtOH, 2 times, 30 sec. each 100% EtOH, 2 times, 30 sec. each Xylenes, 1 min. Xylenes, 1 min. Coverslip with Accumount medium.Reagents Needed: 3L 1x PBS 10mM Tris
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
(Vector Laboratories PK 6100) (optional, see Step 30) Wash solution 1 Wash solution 2 Equipment Aluminum foil Diamond pen Eye protection (see Step 21) Filter paper (e.g., Whatman 3MM) Humid chamber for slide
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