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文献和实验consists of a cooperation between monocytes and cytophilic antibodies able to bind to the Fc receptors present on the monocyte surface. Thus human IgG1 and IgG3 are the main isotypes effective in ADCI, whereas IgG2, IgG4, and IgM are ineffective
Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
Mouse IgG2a isotype control (0.5 mg/ml; BD Pharmingen, cat. no. 554121) Mouse IgM isotype control (0.5 mg/ml; BD Pharmingen, cat. no. 553472
Preparation of Sonicated Human DNA
Purpose: To break up high molecular weight human placental DNA into fragment sizes of 500 bp or less which can be used as competitor DNA in Southern and in situ hybridizations. This method can also be used for sonicating salmon
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