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- 文献和实验
- 技术资料
- 供应商:
合肥瑞辕生物科技有限公司
- 保修期:
Supor® 200 Membrane Disc Filters, 0.2 µm - 47 mm, plain (100/pkg)
- 规格:
100 pkg
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文献和实验Overgo Probing of High-Density Filters
G50 columns. Denature probes before adding to blots. OLB(-A,-C,-N6 ) = A:B:C (1:2.5:1.5) Solution O 1.25M Tris-HCL, pH8 125mM MgCl2 Solution A 1 ml solution O 18µl 2-mercaptoethanol 5µl 0.1M dTTP 5µl 0.1M dGTP Solution B 2M HEPES-NaOH
Preparation of Brain Membrane Fractions
in approximately 5 ml water. Add sucrose and water to give a final volume of 45 ml 1.2 M sucrose, add HEPES to 5 mM and the protease inhibitors as above. Homogenize in glass FEP polymer-homogenizer (3 strokes, 1000 rpm).Spin at 9200 x g for 20 min.Resuspend pellets
MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol
, phosphate-buffered saline, or other buffer at approximately 1 x 106 cells/mL. 2) For the control tube, add 1 μL of 50 mM CCCP (supplied with the kit, 50 μM final concentration) and incubate the cells at 37°C for 5 minutes.Note: CCCP
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