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- 文献和实验
- 技术资料
- 英文名：
Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A)?- Lightning-Link?
- 供应商：
合肥瑞辕生物科技有限公司
- 规格：
3x100ug
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文献和实验Fastfilter Plasmid Maxi Kit Spin Protocol
Column into a 50 ml collection tube, provided. Add 5 ml of Buffer GPS to the column and Lit it sit at room temperature for 3-10 min. Spin in a swinging bucket rotor at 3,000-5,000 x g for 5 minutes at room temperature. Discard the eluate and assemble
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
Use of In Vivo Biotinylation for Chromatin Immunoprecipitation
arrays, or high‐throughput sequencing. (B ) Epitope tagging by in vivo biotinylation. Two recombinant constructs are co‐expressed in the cells: BAP‐X, comprising the protein of interest X fused to a minimal biotin acceptor peptide (BAP, sequence shown
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