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- 文献和实验
- 技术资料
- 供应商：
合肥瑞辕生物科技有限公司
- 保修期：
Finnpipette F2 0.2-2 μl micro, CE marked
- 规格：
1pcs
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文献和实验AFLP: not only for fingerprinting, but for positional cloning
homozygous mutant F2s and collect seed for future use. It is also helpful to save a few non-mutant F2s for tissue collection and F3 seed collection as they may be useful controls for future experiments. 1.1 Isolate genomic DNA NOTE: Use any mini(micro
nucleic acid from mini(micro)prep) 5 U EcoRI 5 U MseI 8.0 µL 5x-Pharmacia "One-Phor-All+" buffer [10x = 100 mM Tris-acetate, pH 7.5, 100 mM Mg-acetate, 500 mM K-acetate) w/ 250 ng/µg BSA (=5x OPA+- BSA) Q.S. to 40 µL w/ dH20 - incubate
AFLP: not only for fingerprinting, but for positional cloning
controls for future experiments. 1.1 Isolate genomic DNA NOTE: Use any mini(micro)prep that yields good quality DNA. CsCl-purified DNA is not necessary. The main contaminants of concern are carbohydrates. If the A260/A230 is _ 2.2 the nucleic acid
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