C1Q Mouse

Classical Complement Pathway Component C1q: Purification of Human C1q, Isolation of C1q Collagen-Like and Globular Head Fragments and Production of Re

The classical complement pathway (CCP) activation is a multimolecular complex, composed of three subcomponents namely C1q, C1r, and C1s. C1q is the recognition subunit of this complex and its binding to the specific targets leads

Purification of C1q Receptors and Functional Analysis

The recognition subunit of C1, C1q, has emerged as an important player in various pathophysiologic conditions largely in part due to its ability to interact with pathogen-associated or cell surface expressed ligands and receptors

螯合沉淀法提取C1q

沉淀，用以上缓冲液洗一次，然后将沉淀物溶于32ml pH值5.0、0.02mol/L醋酸盐缓冲液中； 3. 将以上配制的溶液用4000ml pH值5.0、0.06mol/L EDTA(电导率相当于0.065mol/L NaCl)，于冷环境下，再透析4h，收集沉淀再洗一次，将沉淀溶解于32ml pH值7.5、0.005mol/L PB中，离心使之澄清； 4. 用pH值7.5、0.035mol/L EDTA冷透析5h，使C1q再沉淀，收集沉淀，洗涤后溶解在16ml pH