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文献和实验Human Alkaline Phosphatase staining of transgenic embryo/tissue
. Wash in AP Buffer for 10 minutes. Stain with BM Purple AP Substrate at 4oC for 0.5 to 36 hours (N.B. Incubation at RT will give a quicker result but may result in seepage of staining. Also: NBT/BCIP AP staining has been tried with 9.5dpc embryos
Human Alkaline Phosphatase staining of transgenic embryo/tissue
phosphatase activity by incubation in PBS at 70-75o C for 30 minutes. Rinse in PBS at RT. Wash in AP Buffer for 10 minutes. Stain with BM Purple AP Substrate at 4o C for 0.5 to 36 hours (N.B. Incubation at RT will give a quicker result
). 2. Stacking buffer (4×): 0.5 M Tris-HCl, pH 6.8, and 0.4%(w/v) SDS. Store at RT. 3. 30% acrylamide/bis solution (37.5:1 with 2.6% C) andN , N , N , N ￠-tetramethyl-ethylenediamine (TEMED; Bio-Rad,Hercules, CA). 4. Ammonium persulfate: prepare 10% (w/v
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