MKL-A666728-100μg,Anti-Ang2 &

v468 chapter 5 糖原合成酶激酶-3 的抑制

for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin

Stripping Western Blots

; the more the better) with 0.25-0.5 μg anti-PAK antibody per 100 μg of lysate for 1.5hrs at 4oC with gentle rocking. 6. Add 25 μl protein A-, protein G-, or protein A/G-beads and incubate for 30min at 4oC with gentle rocking. 7. During this incubation, make

v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测

temperature. 4. Permeabilization solution: 0.2% (v/v) Triton X-100 in PBS. 5. Blocking solution: 3% (w/v) albumin from bovine serum(BSA). 6. The first, second, and third antibody solution are preparedin blocking solution. 7. Second antibody