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- 文献和实验
- 技术资料
- 库存:
0
- 英文名:
Transcription Factor E3 Rabbit mAb
- CAS号:
NONE22830
- 供应商:
上海麦克林生化科技股份有限公司
- 保存条件:
-20°C
- 规格:
50μl
商品名称: Transcription Factor E3 Rabbit mAb
商品中文名称: Transcription Factor E3 Rabbit mAb
商品英文名称: Transcription Factor E3 Rabbit mAb
商品CAS: NONE22830
商品型号: T715825
商品货号: MKL-T715825-50μl
相关规格: 50μl,100μl
商品货期: 预计交期:8-10天
商品纯度: 无
商品分子式: 无
商品分子量: 无
储存条件: -20°C
运输条件: 湿冰

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文献和实验Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
). The cells were incubated according to the experimental procedure, the supernatant was removed, and the cells were lysed by the Somatic cell ATP releasing reagent (Sigma-Aldrich). Then, 50 μl of lysate was mixed with 20 μl of ATP reagent containing
Dual- and Triple-Color Fluorospot
100 μl/well of the mixed mAb solution and incubate overnight at 4–8°C (seeNote 1). c. After coating, empty the plate and wash five times with sterile PBS (200 μl/well) to remove excess mAb. Block the plate for at least 30 min with 200 μl
by the Bradford method. 5. Mix 50 μg of proteins with 30 μL of 2× SDS-gel samplebuffer and then boil for 5 min. 3.2. Sds-page 1. These instructions assume the use of a Hoeffer SE-600 gelsystem. 2. Prepare a 1.5-mm thick, 10% gel by mixing 8.0 mL of 4×
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