MKL-C775892-500ml,Cell Stainin

缓冲液配制指南

Buffer 配方 1.0 mol/L Tris•HCl（pH 6.8） 溶解后，用浓盐酸调 pH 至 6.8，最后用超纯水定容至 250 mL，高温灭菌后室温下保存。 1.5 mol/L Tris•HCl（pH 8.8） 溶解后，用浓盐酸调 pH 至 8.8，最后用超纯水定容至 250mL，高温灭菌后室温下保存。 10% SDS 取 SDS 10g，加蒸馏水至 100 mL， 50 ℃ 水浴下溶解，室温保存。如在长期保存中出现沉淀，水浴溶化后，仍可使用。 10% 过硫酸胺（APS） 取

常用缓冲液配制

. Klenow Dilute to 1 unit Klewnow fragment by adding klewnow buffer. Stock Klenow from BRL comes as 500 units in 84 µl. Dilute to 1 unit by adding 420 µl of klenow buffer to the 500 unit/84 µl stock. Store - 20 C. Klenow buffer (250 ml

A general protocol for staining cell for cytometry analysis

General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer