MKL-H960416-5mg,HA Peptide TFA

secondary antibody review -- data from 99 publications

data

peptide fingerprint mapping

prior to rehydration with enzyme.After digestion,the peptides are extracted from the gel and mass analyzed.In-gel digestion procedureMaterials25 mM NH4HCO3 in 50% acetonitrile25 mM NH4HCO3,pH 85% TFA/ 50% acetonitrile0.1 mg/ml trypsin (sequence grade,Promega)in 25 mM

In-gel digestion of proteins for peptide fingerprint mapping

and more sensitive alternative, with the development of ESI-MS and MALDI-MS. Peptide mass fingerprinting uses the peptide masses obtained by digestion to search the protein and DNA databases, to find proteins that show a similar theoretical digest pattern