I17508-5G默克Merck Sigma-Aldrich

Use of a Novel 5′‐Regioselective Phosphitylating Reagent for One‐Pot Synthesis of Nucleoside 5′‐Triphosphates from Unprotected Nucleosides

: Adenosine (5a ) or 2′‐deoxyadenosine monohydrate (4a ) (Sigma‐Aldrich) Cytidine (5c ), 2′‐deoxycytidine monohydrochloride (4c ), guanosine (5g ), 2′

DNA Sequencing

the mixture to ~40℃, with stirring, to dissolve solids. N.B.: Do not heat the mixture above 55℃ as this leads to hydrolysis of the urea. 3) Once dissolved, add ~5g Amberlite MB-1 resin and stir for 20 minutes. 4) Filter solution (we typically use Whatman

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

RF2 50ml RbCl 60mg MOPS 1ml 0.5M pH6.8 (adjust w/ NaOH) CaCl2 2H2O 0.55g Glycerol 7.5g Adjust to pH 6.8 w/ NaOH. Filter through 0.22um. Store RT   E COLI TRANSFORMATION 1) thaw competent cells (-70 C stored) on ice