I6256-500MG默克Merck Sigma-Aldri

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

dilutions for rabbit polyclonal antibodies range between 1:50 and 1:500. For double-labeling experiments, use primary antibodies raised in two different species. Antibodies, secondary Secondary antibodies are diluted in PBS containing 2% normal

植物蛋白质组学中的双向电泳技术

- Aldrich) 和 2.0 g SDS (Serva) 溶解于约 400 ml 去离子水中。 b. 用 4 mol/L HCl ( Merck) 调整 pH 至 8.6 并用去离子水定容至 500 ml。 c. 加入 50 mg 叠氮化钠（Merck) 并过滤。缓冲液可在 4°C 保存 2 周。 ( 5 ) 电极缓冲液储液：配制 5 L 电极缓冲液储液：溶解 58.0 g TriZma 碱（SigmaAldrich) ，299.6 g 甘氨酸（ Sigma- Aldrich) 和 19

COMPARISION OF DIFFERENT PROTEIN DETERMINATION METHODS

, Ammonium sulphate, Sulphydryl compounds, PO4 buffers Folin-Ciocalteu reagent: SIGMA (#F9252) - MERCK LOWRY 10 µg/ml - 1 mg/ml Very accurate 1 ml reagent + 0.2ml sample