T8625-10G默克Merck Sigma-Aldrich

Synthesis of Building Blocks and Oligonucleotides with {G}O6‐Alkyl‐O6{G} Cross‐Links

. Nucleic Acid Chem. 44:5.9.1?5.9.19. © 2011 by John Wiley & Sons, Inc. Keywords: interstrand cross?link; phosphoramidite; oligonucleotide synthesis; chemical synthesis; solid?phase synthesis; DNA repair      

Appendix G: Spectrophotometry

of the transmittance.   A = - log T EQUATION G.1 This value is more useful in spectrophotometry than transmittance, because of plot of absorbance vs concentration yields a straight line. A plot of transmittance vs

TOP10 chemically competent cells

or 10-5 μg/μl This can be made by diluting 1 μl of NEB pUC19 plasmid (1 μg/μl, NEB part number N3401S) into 100 ml of TE Hold on ice 0.5 hours Heat shock 60 sec at 42C Add 250 μl SOC Incubate at 37 C for 1 hour