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- 文献和实验
- 技术资料
- 库存:
期货
- 英文名:
Biotin-16-dCTP
- CAS号:
2567941-52-8
- 供应商:
上海阿拉丁生化科技股份有限公司
- 保存条件:
-20°C储存;超低温运输
- 规格:
B1424431-1mg
中文名:Biotin-16-dCTP
英文名:Biotin-16-dCTP
货号:B1424431
Cas号:2567941-52-8
存储温度:-20°C储存
运输条件:超低温运输
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文献和实验PRINS for Mapping Single-Copy Genes
-specific oligonucleotide probes (PRINS primers) were annealed to chromosomal DNA in situ and extended on preparations fixed on glass slides in the presence of dATP, dCTP, dGTP, dTTP, biotin-16-dUTP, and Taq DNA polymerase. After addition of avidin
in a microcentrifuge. 5.Incubate at room temperature (25 degrees Celsius) for 1 hour. 6.Add 2 μl of stop buffer. To purify and/or determine the activity of incorporated dCTP- continue. 7.Dilute 2 μl of the 52 μl sample into 500 μl with 498 μl of distilled water
5.Incubate at room temperature (25 degrees Celsius) for 1 hour. 6.Add 2 μl of stop buffer. To purify and/or determine the activity of incorporated dCTP- continue. 7.Dilute 2 μl of the 52 μl sample into 500 μl with 498 μl of distilled
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