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- 文献和实验
- 技术资料
- 库存:
现货
- 现货状态:
现货
- 供应商:
上海然其生物科技有限公司
- 规格:
10个/包/28包/箱
| 规格: | 10个/包 | 产品价格: | ¥35.96 |
|---|---|---|---|
| 规格: | 28包/箱 | 产品价格: | ¥1007.0 |
Nunc™ EasYDish™ 60 mm 细胞培养皿 货号:150462
中文产品介绍
产品简介
Nunc EasYDish 60 mm 细胞培养皿,采用Nunclon™ Δ 等离子 TC 贴壁处理表面,高透明聚苯乙烯材质,γ 射线辐照灭菌;无 DNase/RNase、无热原、无细胞毒性,符合 USP Class‑VI 生物安全标准Thermo Fis...。皿盖带有通风缺口,保障 CO₂气体交换,适合中等规模贴壁细胞传代、克隆形成、药物处理与显微成像实验。
- 货号:150462
- 直径:60 mm
- 培养面积:21.5 cm²
- 皿高:16 mm
- 推荐培养基体积:5‑7 mL
- 包装规格:10 个 / 内袋,280 个 / 整箱Thermo Fis...
- 灭菌:γ 射线辐照灭菌(SAL 10⁻⁶)
主要特点
- Nunclon™ Δ 等离子表面处理:对肿瘤细胞、原代细胞均具备稳定贴壁效果,批间一致性优异。
- 皿盖缺口通风结构:兼顾无菌性能与孵箱内气体交换,限制皿盖移位,提升机械强度Thermo Fis...。
- 斜角防滑握环,戴手套操作不易滑脱;皿底四向定位标记,方便显微镜下定位细胞;皿边设置书写区,便于样本标记追溯Thermo Fis...。
- 皿盖凸起外缘,堆叠稳固,节省培养箱空间;高透明平底,适配明场、荧光显微观察。
- 包装袋可重新密封;一次性使用,禁止高压灭菌;无动物源组分Thermo Fis...。
下游应用
贴壁细胞传代扩增,克隆形成实验,细胞转染,药物干预实验,荧光显微成像,中等规模原代细胞培养。
储存条件
室温干燥避光存放,远离高温;不可高压灭菌。
⚠️提示:本品为哺乳动物细胞 TC 处理培养皿,不适合细菌真菌微生物培养。
English Product Introduction
Product Description
Nunc™ EasYDish™ 60 mm cell‑culture dish (Cat.No.150462), Nunclon™ Δ plasma‑treated tissue‑culture surface. Fabricated from optically‑clear polystyrene, gamma‑irradiated sterile, DNase‑/RNase‑free, non‑pyrogenic, non‑cytotoxic and USP Class‑VI compliantThermo Fis.... Notched‑lid design allows sufficient gas exchange in CO₂ incubator. Suitable for medium‑scale adherent cell subculture, colony‑formation assay, drug treatment and microscopic imaging.
- Catalog No.: 150462
- Diameter: 60 mm
- Growth area: 21.5 cm²
- Height:16 mm
- Recommended working volume: 5‑7 mL medium
- Pack size: 10 dishes per inner sleeve, total 280 per caseThermo Fis...
- Sterilization: Gamma irradiation (SAL 10⁻⁶)
Key Features
- Nunclon™ Δ surface: Stable cell‑attachment performance for cell lines and primary cells, excellent lot‑to‑lot consistency.
- Notched vented‑lid: maintains sterility while enabling gas exchange, restricts lid displacement and improves mechanical strengthThermo Fis....
- Beveled grip ring for safe gloved‑hand operation; orientation markers on dish bottom facilitate cell positioning under microscope; writing area for sample traceabilityThermo Fis....
- Raised lid rim ensures stable stacking in incubator; optically‑clear flat bottom compatible with bright‑field and fluorescence microscopy.
- Resealable outer bag; single‑use only, do not autoclave. Animal‑component‑free raw materialsThermo Fis....
Applications
Adherent‑cell sub‑cultivation, colony‑formation assay, cell transfection, drug‑treatment experiments, fluorescence microscopy, medium‑scale primary‑cell culture.
Storage
Store at room‑temperature, dry and dark environment. Do not autoclave.
Note: TC‑treated for mammalian cell culture, not for bacterial or fungal microbiological culture.
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文献和实验- Cell Biology International,2023,肿瘤细胞克隆形成实验
Human cancer cell lines were seeded in Nunc 60‑mm tissue‑culture‑treated dishes for colony‑formation assays.
- FEBS Open Bio,2022,原代细胞药物处理
Primary mouse cells were maintained in Nunc 60 mm Nunclon‑Δ cell‑culture dishes for compound exposure experiments.
- 中文核心《细胞生物学杂志》
贴壁细胞接种于 Nunc 60 mm 细胞培养皿,用于细胞转染与荧光显微观察。
CCMB buffer to make chemical competent cell
at 23 C room temperature works well Add glycerol to 15% Aliquot 1 ml samples to Nunc cryotubes Place tubes into a zip lock bag, immerse bag into a dry ice/ethanol bath for 5 minutes This step may not be necessary Place in -80 freezer indefinitely
Methylated CpG Island Amplification
Protocol written by Minoru Toyota 1. Materials 1.1. MCA Restriction enzymes SmaI, XmaI T4 DNA ligase Taq DNA polymerase 10X PCR reaction buffer: 670mM Tris-HCl, pH 8.8 40mM MgCl2 160 mM NH
,经37℃保温60分钟后在367/455M条件下测定相对荧光强度,再从事先绘制的NADPH荧光测定工作曲线上读出NADPH含量。l单位(U)酶活性表示一分钟内1ummlo NADPH被氧化的酶量,每份标本的AR活性用U/gHb表示。ELISA法:血约1m1置肝家防凝管,10叨8离心15分钟,吸去血清后红细跑用3倍体积的磷酸盐/氯化钠溶液(100mmol/L磷酸钾/145mm0l/l氯化钠,v/v=9:1)冲洗三次,加等体积10mmol/l的磷酸缓冲液(ph 7.0)在15000g离心15分钟,弃去沉淀
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