Anti-GFAP 抗体 [EPR1034Y]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR1034Y

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

IHC-Fr, IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra), mIHC

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/500 - 1/2000", "mIHC-species-notes": "

", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "

", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "

", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/500 - 1/2000", "mIHC-species-notes": "

", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "

", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.1-1 µg/mL", "ICCIF-species-notes": "

", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "

", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250 - 1/1000", "IHCFr-species-notes": "

" }, "Rat": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/500 - 1/2000", "mIHC-species-notes": "

", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.1-1 µg/mL", "ICCIF-species-notes": "

", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/40", "IP-species-notes": "

", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "

", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/250 - 1/1000", "IHCFr-species-notes": "

" } } }

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

• Visit the General protocols
• Visit the Troubleshooting

产品详情

Find all reagents to label astrocytes in our "Astrocytes markers guide".

Anti-GFAP antibody [EPR1034Y] (ab68428) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, IP and WB.

Anti-GFAP antibody [EPR1034Y] (ab68428) was first used in a scientific publication in 2011 and has been cited over 106 times in peer reviewed journals. It's performance in IHC in mouse samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-GFAP antibody [EPR1034Y] (ab68428) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-GFAP antibody [EPR1034Y] (ab68428) has 15 independent reviews from customers.

Anti-GFAP antibody [EPR1034Y] (ab68428) specifically detects GFAP (UniProt ID: P14136; Molecular weight: 50kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Conjugation-ready, carrier free format available for antibody clone EPR1034Y - ab218309.

Antibody clone EPR1034Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor^® 488, Alexa Fluor^® 647, Alexa Fluor^® 594, Alexa Fluor^® 555, Alexa Fluor^® 568, Alexa Fluor^® 405, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor^® 647, Biotin, Alexa Fluor^® 488, Alexa Fluor^® 647, Alexa Fluor^® 647, Alexa Fluor^® 488, Alexa Fluor^® 488 (ab194324, ab194325, ab201732, ab201735, ab201736, ab206586, ab303005, ab303006, ab303007, ab308965, ab313596, ab314264, ab317669, ab317720, ab317721, ab317762, ab319022).

NEW: Explore our alternative host species for clone [EPR1034Y] : goat, chicken, rat, mouse. GFAP (Glial Fibrillary Acidic Protein) is a key marker in neuro research, particularly for its role in identifying astrocyte activity. It is predominantly expressed in astrocytes, the most abundant glial cells in the brain and spinal cord. Altered GFAP levels are associated with various neurological conditions, including Alzheimer's disease (AD), traumatic brain injury (TBI) and multiple sclerosis (MS). Researchers use GFAP to assess astrocytic response and neuroinflammation, making it essential for understanding the extent of brain injury and the progression of neurodegenerative diseases.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

• - High batch-to-batch consistency and reproducibility
• - Improved sensitivity and specificity
• - Long-term security of supply
• - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA

运输条件

Conditional Ambient

推荐的短期储存时间

1-2 weeks

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

靶点信息

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

See full target information GFAP