重组Anti-LAG-3抗体[EPR20294-77]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20294-77

亚型

IgG

不含载体蛋白

No

反应种属

Mouse

应用

IP, WB, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "

", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "

", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "

" } } }

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

• Visit the General protocols
• Visit the Troubleshooting

产品详情

What is this antibody validated in?
Anti-LAG-3 antibody [EPR20294-77] (ab209238) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

What is the molecular weight of LAG-3?
Anti-LAG-3 [EPR20294-77] (ab209238) specifically detects a band for LAG-3 (UniProt: Q61790) at a molecular weight of 57kDa.

Other related products
We have a range of other formats of antibody clone [EPR20294-77] also available for your convenience: ab209238, Carrier free - ab236154

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

• - High batch-to-batch consistency and reproducibility
• - Improved sensitivity and specificity
• - Long-term security of supply
• - Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

推荐的短期储存时间

1-2 weeks

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

靶点信息

Lymphocyte activation gene 3 protein. Inhibitory receptor on antigen activated T-cells (PubMed : 12209638, PubMed : 12421911, PubMed : 12672063, PubMed : 15100286, PubMed : 15634887, PubMed : 30580966, PubMed : 35413245, PubMed : 35761082, PubMed : 38483996, PubMed : 39209860). Delivers inhibitory signals upon binding to ligands, such as MHC class II, its main ligand present at the surface of antigen-presenting cells (APCs), and FGL1, which is secreted by hepatocytes and certain types of tumor cells (PubMed : 12209638, PubMed : 12421911, PubMed : 30349037, PubMed : 30580966, PubMed : 35413245, PubMed : 35761082, PubMed : 39209860). Ligand-binding initiates a signaling that inhibits the T-cell receptor (TCR) in the immunological synapse, preventing T-cell activation (PubMed : 12209638, PubMed : 12421911, PubMed : 12672063, PubMed : 15100286, PubMed : 15634887). Mechanistically, ligand-binding promotes (1) ubiquitination of the KIEELE motif, unleashing the RRFSALE motif from the membrane and (2) leading to the formation of condensates with the TCR component CD3E, thereby disrupting the association between CD3E and LCK and preventing TCR activation (PubMed : 40101708, PubMed : 40592325). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1 (PubMed : 21300912). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed : 12209638, PubMed : 12421911, PubMed : 12672063, PubMed : 15100286, PubMed : 15634887). Also mediates immune tolerance : constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (PubMed : 15485628). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (PubMed : 19201841).. The LAG3-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival.. Secreted lymphocyte activation gene 3 protein. May function as a ligand for MHC class II (MHC-II) on antigen-presenting cells (APC), promoting APC activation/maturation and driving Th1 immune response.

See full target information Lag3