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- 文献和实验
- 技术资料
- 免疫原:
详询
- 亚型:
详询
- 形态:
液体
- 保存条件:
-20℃
- 克隆性:
详询
- 标记物:
详询
- 适应物种:
Mouse
- 保质期:
12个月
- 抗原来源:
详询
- 目录编号:
MP369147
- 级别:
科研级
- 库存:
20
- 供应商:
北京百奥创新科技有限公司
- 宿主:
Rat
- 应用范围:
FCM, IF
- 浓度:
1mg/mL
- 靶点:
详询
- 抗体英文名:
Anti-Mouse CD16/CD32 Antibody (2.4G2), PerCP
- 抗体名:
Anti-Mouse CD16/CD32 抗体 (2.4G2), PerCP
- 规格:
50T/100T
产品名称:Anti-Mouse CD16/CD32 Antibody (2.4G2), PerCP
货号:MP369147
反应种属:Mouse
应用实验:FCM, IF
靶点:Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, CD16, Low affinity immunoglobulin gamma Fc region receptor II, Fc gamma receptor IIB, Fc-gamma RII, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Lymphocyte antigen 17, Ly-17, CD32, Fcgr2, Fcgr2b, Ly-17
说明:Anti-Mouse CD16/CD32 Antibody (2.4G2), PerCP [2.4G2] ( MP369147 ) is a rat monoclonal antibody detecting Low affinity immunoglobulin gamma Fc region receptor III in FCM, IF . Suitable for Mouse . Highlights Flow Cytometry — Validated for flow cytometry applications. Fluorescent Label — Direct conjugate for streamlined workflows.
基因号:P08508, P08101

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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD16
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
、ionomycin和Brefeldin A,其中前两者协同激活细胞,后者抑制蛋白转运,从而有效增强胞内细胞因子的积累与检测信号。 03 封闭剂 单核细胞、巨噬细胞和B细胞表面高表达Fc受体,易非特异性结合抗体的Fc段,导致假阳性信号。为解决此问题,菲恩生物提供Purified Anti-Mouse CD16/32 Antibody(FNab30106)用于小鼠样本封闭,;Human Fc Receptor Blocking reagent(K128)用于人样本封闭。关键在于必须选择与样本
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