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- 文献和实验
- 技术资料
- 抗体名:
HRP标记的TATA盒结合蛋白抗体
- 抗体英文名:
Anti-TBP Monoclonal Antibody-HRP
- 靶点:
TBP
- 浓度:
WB 1:1000-3000.
- 应用范围:
WB
- 宿主:
Mouse
- 供应商:
北京索莱宝科技有限公司
- 库存:
999
- 级别:
实验级
- 目录编号:
6908
- 抗原来源:
Recombinant Protein
- 保质期:
Store at -20℃. Avoid freeze / thaw cycles.
- 标记物:
Biotin,Cy3,Cy3.5,Cy5,Cy5.5,Cy7,FITC,HRP,RBITC,SAlexa Fluor 350,SAlexa Fluor 405,SAlexa Fluor 488,SAlexa Fluor 532,SAlexa Fluor 555,SAlexa Fluor 568,SAlexa Fluor 594,SAlexa Fluor 633,SAlexa Fluor 640,SAlexa Fluor 647,SAlexa Fluor 660,SAlexa Fluor 680,SAlexa Fluor 750
- 克隆性:
Monoclonal Antibody
- 保存条件:
Store at -20℃. Avoid freeze / thaw cycles.
- 形态:
干粉/液体
- 亚型:
IgG1
- 规格:
100ul
| 英文名称 | Anti-TBP Monoclonal Antibody-HRP |
| 中文名称 | HRP标记的TATA盒结合蛋白抗体 |
| 规格 | 100ul |
| 宿主 | Mouse |
| 克隆类型 | Monoclonal Antibody |
| 亚型 | IgG1 |
| 交叉反应 | Human ; Mouse ; Rat |
| 应用 | WB |
| 稀释比例 | WB 1:1000-3000. |
| 靶标 | TBP |
| 别名 | GTF2D1; TF2D; TFIID; TATA Binding Protein |
| 背景说明 | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. |
| Swiss Prot | P20226 |
| Gene ID | 6908 |
| 亚细胞定位 | Nucleus |
| 免疫原 | Recombinant Protein |
| 理论分子量 | 38/36kDa |
| 纯化方法 | Affinity purification |
| 储存液 | PBS with 50% Glycerol, 0.03% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20℃. Avoid freeze / thaw cycles. |
| 运输条件 | 冷藏运输 |
| 偶联物 | HRP |
| 单位 | 支 |
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma,在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应,对应于抗原之不同部分可制成各种不同的抗体复合体。然而,因为一个产生抗体的细胞,只能产生一种抗体,所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种,可形成边产生抗体边增殖的 hybridoma,将每个杂种细胞进行克隆(无性繁殖系)培养,则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成,这样得到的就是单克隆抗体。此抗体在测定
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
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