MITF Antibody (N-term) 抗体,orb29533,Biorbyt产品图

MITF Antibody (N-term) 抗体,orb2

9533,Biorbyt
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  • ¥2106 - 3861
  • Biorbyt已认证
  • orb29533
  • 英国
  • 2026年08月07日
  • FC, IF, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      MITF Antibody (N-term) 抗体

    • 抗体英文名

      MITF Antibody (N-term)

    • 靶点

      MITF

    • 应用范围

      FC, IF, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb29533

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型

      Rabbit IgG

    • 免疫原

      详询

    • 规格

      50 ul/100 ul

    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3861.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Peptide affinity purified rabbit polyclonal antibody.

    别名: Anti-Microphthalmia-associated transcription factor, MITF, BHLHE32 antibody

    应用稀释比例: IF - 1:10-50, WB - 1:2000, IHC - 1:800, FC - 1:10-50

    免疫原: Synthetic Peptide

    分子量: 58795

    预测反应性: Mouse, Rat

    研究领域: Immunology & Inflammation

    UniProt ID: O75030

    NCBI 参考序列: NP_000239.1, NP_937820.1, NP_937821.2, NP_937802.1, NP_937801.1, NP_001171896.1, NP_006713.1

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    Anti-MITF Antibody (N-term) at 1:2000 dilution + A375 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片2
    Confocal immunofluorescent analysis of MITF Antibody (N-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

    产品细节图片3
    Immunohistochemical analysis of paraffin-embedded Human Melanoma section using Pink1. diluted at 1:800 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

    产品细节图片4
    MITF Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

    产品细节图片5
    MITF Antibody (N-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the MITF antibody detected the MITF protein (arrow).

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    图标文献和实验
    相关实验
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      In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important

    • Antibody Storage

      to protect the antibody. These should be stored in smaller aliquots (about 1 ml) and frozen for long term storage though the protected solution is also stable for months at four degrees. Ascites fluid: This is a bit more problematic as it may contain

    • Biotinylation of Antibody

        Materials 0.1M NaHC03 pH9 DMSO NHS-Biotin (N-Hydroxysuccinimidobiotin, Sigma #H-1759) PBS Procedure Dialyze the sample against carbonate buffer. After dialysis, adjust

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    MITF Antibody (N-term) 抗体,orb29533,Biorbyt
    ¥2106 - 3861