RBP4 Antibody / Retinol Binding Protein 4 抗体,orb2644806,Biorbyt产品图

RBP4 Antibody / Retinol Bindin

g Protein 4 抗体,orb2644806,Biorbyt
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  • ¥8775
  • Biorbyt已认证
  • orb2644806
  • 英国
  • 2026年08月07日
  • IHC-P, WB
  • Mouse
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      RBP4 Antibody / Retinol Binding Protein 4 抗体

    • 抗体英文名

      RBP4 Antibody / Retinol Binding Protein 4

    • 应用范围

      IHC-P, WB

    • 宿主

      Mouse

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb2644806

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Monoclonal

    • 保存条件

      详询

    • 亚型

      Mouse IgG2b, kappa

    • 免疫原

      详询

    • 规格

      100 ug

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Retinol (Vitamin A) is transported in the blood bound to its carrier protein, retinol-binding protein (RBP), also designated plasma retinol-binding protein (PRBP) or RBP4. A member of the lipocalin family, RBP conveys retinol from stores in the liver to peripheral tissues. In plasma, RBP binds transthyretin (TTR, formerly called prealbumin) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The stability of this complex holds diagnostic importance because the molar ratio of RBP:TTR provides an indirect way to indicate marginal Vitamin A deficiency. Vitamin A deficiency blocks the secretion of RBP, resulting in defective delivery and supply to epidermal cells. Originally identified solely as a transporter protein, recent studies correlating increased levels of RBP expression in adipose tissue with Insulin resistance have generated research into the possible roles the protein may play in the pathogenesis of type 2 diabetes and obesity.

    克隆号: RBP4/4314

    应用稀释比例: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT

    应用说明: Optimal dilution of the RBP4 antibody should be determined by the researcher.

    免疫原: A portion of amino acids 29-148 from the human protein was used as the immunogen for the RBP4 antibody.

    防腐剂: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

    纯化方法: Protein G affinity chromatography

    研究领域: Pharmacology & Drug Discovery

    UniProt ID: P02753

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot testing of human pancreas tissue with RBP4 antibody. Predicted molecular weight ~23 kDa.

    产品细节图片2
    Western blot testing of human 1) liver and 2) kidney tissue with RBP4 antibody. Predicted molecular weight ~23 kDa.

    产品细节图片3
    IHC staining of FFPE human kidney with RBP4 antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

    产品细节图片4
    Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using RBP4 antibody. These results demonstrate the foremost specificity of the RBP4/4314 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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    图标文献和实验
    相关实验
    • Protein A Purification of Antibody

      1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M

    • Protein A Purification of Antibody

        Reagents   Affi-gel Protein-A Agarose (BioRad #153-6153)   MAPS II Binding Buffer (BioRad # 153-6161) 0.314 g/ml diH2 O MAPS II Elution Buffer (BioRad #153-6162)

    • Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction

      ?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps

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