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- 文献和实验
- 技术资料
- 抗体名:
RBP4 Antibody / Retinol Binding Protein 4 抗体
- 抗体英文名:
RBP4 Antibody / Retinol Binding Protein 4
- 应用范围:
IHC-P, WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb2644806
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 亚型:
Mouse IgG2b, kappa
- 免疫原:
详询
- 规格:
100 ug
产品子类型: Primary Antibody
描述: Retinol (Vitamin A) is transported in the blood bound to its carrier protein, retinol-binding protein (RBP), also designated plasma retinol-binding protein (PRBP) or RBP4. A member of the lipocalin family, RBP conveys retinol from stores in the liver to peripheral tissues. In plasma, RBP binds transthyretin (TTR, formerly called prealbumin) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The stability of this complex holds diagnostic importance because the molar ratio of RBP:TTR provides an indirect way to indicate marginal Vitamin A deficiency. Vitamin A deficiency blocks the secretion of RBP, resulting in defective delivery and supply to epidermal cells. Originally identified solely as a transporter protein, recent studies correlating increased levels of RBP expression in adipose tissue with Insulin resistance have generated research into the possible roles the protein may play in the pathogenesis of type 2 diabetes and obesity.
克隆号: RBP4/4314
应用稀释比例: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
应用说明: Optimal dilution of the RBP4 antibody should be determined by the researcher.
免疫原: A portion of amino acids 29-148 from the human protein was used as the immunogen for the RBP4 antibody.
防腐剂: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
纯化方法: Protein G affinity chromatography
研究领域: Pharmacology & Drug Discovery
UniProt ID: P02753
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.




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文献和实验Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
Protein A Purification of Antibody
Reagents Affi-gel Protein-A Agarose (BioRad #153-6153) MAPS II Binding Buffer (BioRad # 153-6161) 0.314 g/ml diH2 O MAPS II Elution Buffer (BioRad #153-6162)
Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps
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