- ￥8775
- Biorbyt
- orb2644754
- 英国
- 2026年08月07日
- IHC-P, WB
- Mouse
- Human
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- 文献和实验
- 技术资料
- 抗体名：
Myelin Basic Protein Antibody 抗体
- 抗体英文名：
Myelin Basic Protein Antibody
- 应用范围：
IHC-P, WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb2644754
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG2a
- 免疫原：
详询
- 规格：
100 ug
产品子类型: Primary Antibody
描述: Myelin basic protein (MBP) is the second most abundant protein in central nervous system (CNS) myelin: it comprises 30% of the total protein and about 10% of the dry weight of myelin. It is the only structural protein found so far to be essential for formation of CNS myelin, and has been called the executive molecule of myelin . MBP can interact with a number of polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and negatively charged lipids, and acquires structure on binding to them. It may act as a membrane actin-binding protein, which might allow it to participate in transmission of extracellular signals to the cytoskeleton in oligodendrocytes and tight junctions in myelin. MBP may be applicable as a marker for oligodendrogliomas. Phosphorylation been shown to decrease its ability to aggregate lipid vesicles and consequently destabilising the compact structure ofmyelin, a destruction that has been observed in demyelinating diseases such as Multiple Sclerosis. MBP/4276 is a useful detector of phosphorylated MBP by binding to Thr98 of human MBP in the phosphorylated state.
克隆号: MBP/4276
应用稀释比例: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
应用说明: Optimal dilution of the Myelin Basic Protein antibody should be determined by the researcher.
免疫原: A synthetic peptide corresponding to the amino acids surrounding phosphorylated threonine 98 were used as the immunogen for the Myelin Basic Protein antibody.
防腐剂: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
纯化方法: Protein G affinity chromatography
研究领域: Neuroscience
UniProt ID: P02686
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验The COS-7 Cell In Vitro Paradigm to Study Myelin Proteolipid Protein 1 Gene Mutations
Despite many shortcomings, a reductionist approach using cell culture paradigms to define basic principles underlying disease processes has considerable merit. One example of the utility of this approach is the expression of mutant forms
There are many different types of ELISAs, which can detect the presence of protein in serum or supernatent. One of the most common types of ELISA is the so-called "sandwich ELISA." It is termed this because the antibody
Basic Method for Indirect Immunofluorescence Labeling
In flow cytometry, fluorescence is relative. We need a negative control to determine where "positivity" begins. Secondary antibody: Fluorochrome-conjugated antibodies directed towards the protein composing the primary
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