- ￥8775
- Biorbyt
- orb2642993
- 英国
- 2026年08月07日
- IF, IHC-P, WB
- Mouse
- Human
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- 技术资料
- 抗体名：
Prohibitin Antibody / PHB 抗体
- 抗体英文名：
Prohibitin Antibody / PHB
- 应用范围：
IF, IHC-P, WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb2642993
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG2b, kappa
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: This antibody recognizes a protein of 30kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
克隆号: PHB/3225
应用稀释比例: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
应用说明: Optimal dilution of the Prohibitin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.
防腐剂: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
纯化方法: Protein G affinity chromatography
UniProt ID: P35232
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: This antibody recognizes a protein of 30kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
克隆号: PHB/3225
应用稀释比例: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
应用说明: Optimal dilution of the Prohibitin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.
防腐剂: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
纯化方法: Protein G affinity chromatography
UniProt ID: P35232
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Immunofluorescence staining of human HepG2 cells with Prohibitin antibody (green, clone PHB/3225) and Reddot nuclear stain (red).
IHC testing of FFPE human liver tissue with Prohibitin antibody (clone PHB/3225). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human liver tissue with Prohibitin antibody (clone PHB/3225). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Prohibitin antibody (clone PHB/3225). These results demonstrate the foremost specificity of the PHB/3225 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free Prohibitin antibody (clone PHB/3225) as confirmation of integrity and purity.
Western blot testing of human liver lysate with Prohibitin antibody (clone PHB/3225). Expected molecular weight ~30 kDa.
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Prohibitin Antibody / PHB 抗体，orb2642993，Biorbyt
￥8775
