- ￥8775
- Biorbyt
- orb2638321
- 英国
- 2026年08月07日
- IHC-P
- Mouse
- Human, Rat
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- 文献和实验
- 技术资料
- 抗体名：
Calponin Antibody Cocktail 抗体
- 抗体英文名：
Calponin Antibody Cocktail
- 应用范围：
IHC-P
- 宿主：
Mouse
- 适应物种：
Human, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb2638321
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG1, kappa
- 免疫原：
详询
- 规格：
7 ml
产品子类型: Primary Antibody
描述: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
克隆号: CNN1/832 + CALP
应用稀释比例: The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
应用说明: Optimal dilution of the Calponin antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: Recombinant full-length human protein (CNN1/832) and crude human uterus extract (CALP) were used as the immunogen for the Calponin antibody cocktail.
防腐剂: Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
纯化方法: Protein G affinity chromatography
研究领域: Musculoskeletal & Connective Tissue Research
UniProt ID: P51911
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验人钙调蛋白 (Calponin)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 Calponin 单抗包被于酶标板上，标准品和样品中的 Calponin与单抗结合，加入生物素化的抗人 Calponin ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合，加入底物工作液显蓝
实验步骤 1. Measure out the amount of Taq DNA polymerase needed. When using a reaction cocktail, determine the total number of units in the mix. 2. Add an amount of “equivalent units” of Platinum® Taq
Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps
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