- ￥3550
- AGRISERA
- 瑞典
- AS07 214
- 2026年08月07日
- WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- Rabbit
- Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
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- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型：
Rabbit IgG
- 形态：
Lyophilized
- 保存条件：
-20°C.
- 克隆性：
Polyclonal
- 标记物：
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期：
long term storage (1 year or more) at -20°C.
- 抗原来源：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别：
科研
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度：
Concentration after reconstitution: 1 µg/µL
- 抗体英文名：
Baker's yeast replication factor A
- 抗体名：
Anti-RFA
- 规格：
50 µl
Anti-RFA | Baker's yeast replication factor A
AS07 214| Clonality:Polyclonal| Host:Rabbit| Reactivity:Saccharomyces cerevisiae
Product Info
| Immunogen: | RPA from Saccharomyces cerevisiae consisting of three subunits RFA1 (70 kDa), RFA2 (30 kDa) and RFA3 (14 kDa); overexpressed in E.coli and purified by chromatography; no affinity tags were added to any of three subunits |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Serum |
| Format: | Lyophilized |
| Quantity: | 50 µl |
| Reconstitution: | For reconstitution add 50 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Western blot (WB) |
| Recommended dilution: | ChIP, 1 : 20 000 (WB) |
| Expected | apparent MW: | 70 + 30 + 14 kDa |
Reactivity
| Confirmed reactivity: | Saccharomyces cerevisiae |
| Predicted reactivity: | Saccharomyces cerevisiae |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
Additional Information
Additional information (application): Antibody was also successfully used in ChIP application Holstein et al. (2014). Load of 1 ng of the protein will allow to visualize two subunits of RPA, while load of 5 ng will allow to visualize all three subunits in Western blot technique.
Background
Saccharomyces cerevisiae replication protein A (RPA), also known as replication factor A (RFA) is a single-stranded DNA-binding protein that is required for multiple processes in eukaryotic DNA metabolism. Those processes include DNA replication, DNA repair, and recombination. Homologues to RPA have been identified in all eukaryotic organisms examined. RPA is heterotrimeric protein composed of subunits of approximately 70, 30, and 14 kDa. Members of this family bind nonspecifically to single-stranded DNA and interact with and/or modify the activities of multiple proteins. Alternative names: Replication protein A 69 kDa DNA-binding subunit, Single-stranded DNA-binding protein, DNA-binding protein BUF2, replication protein A 36 kDa subunit, DNA-binding protein BUF1 antibody
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文献和实验Replication timing by density transfer
in Ycomp+[12 C]-glucose+ alpha factor. [I don't particularly try to resuspend the cells in exactly the same volume they were in originally, but rather, in a volume that's convenient.] 4. Transfer to 37o C, wait for culture to reach 37o C
Single-molecule analysis of DNA replication by molecular combing
The protocol described below is meant to detect BrdU incorporation in yeast cells arrested in early S phase with hydroxyurea (HU). It can be easily adapted to monitor ongoing DNA replication in asynchronous yeast cultures or to analyze DNA replication
Replication timing using transient hemimethylation
of replication initiation in yeast chromosomes J. Cell Sci. Suppl. 19:51-58. Rationale of methylation timing approach The E. coli dam methylase adds methyl groups to adenine in the sequence 5' GATC 3' ( Marinus and Morris, 1973
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