- ￥1940
- AGRISERA
- 瑞典
- AS10 858
- 2026年08月07日
- WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- Rabbit
- Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
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- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型：
Rabbit IgG
- 形态：
Lyophilized
- 保存条件：
-20°C.
- 克隆性：
Polyclonal
- 标记物：
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期：
long term storage (1 year or more) at -20°C.
- 抗原来源：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别：
科研
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度：
Concentration after reconstitution: 1 µg/µL
- 抗体英文名：
Rabbit IgG Fc, HRP conjugated
- 抗体名：
Goat anti-Rabbit IgG Fc, HRP conjugated
- 规格：
1 mg
Goat anti-Rabbit IgG Fc, HRP conjugated
AS10 858| Clonality:Polyclonal| Host:Goat| Reactivity:Rabbit IgG Fc (two heavy chains with constant domains)
Product Info
| Immunogen: | Purified Rabbit IgG |
| Host: | Goat |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified goat IgG. |
| Format: | Lyophilized |
| Quantity: | 1 mg |
| Reconstitution: | For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily |
| Storage: | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
| Tested applications: | ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB) |
| Recommended dilution: | The optimal working dilution should be determined by the investigator |
Reactivity
| Confirmed reactivity: | Rabbit IgG Fc (two heavy chains with constant domains) |
| Predicted reactivity: | Rabbit IgG Fc (two Heavy chains with constant domains) |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
Additional Information
HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free 0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase Additional information (application): This antibody reacts with the heavy chains on rabbit IgG based on immunoelectrophoresis. No reactivity is observed to the light chains on rabbit immunoglobulins or non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis. This antibody will cross react with the heavy chain on other species IgG, but not with IgM or IgA.
Background
Goat anti-rabbit IgG Fc (two heavy chains with constant domains) is a secondary antibody conjugated to HRP which binds to rabbit IgG Fc (two heavy chains with constant domains) in immunological assays .
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Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
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