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- AGRISERA
- 瑞典
- AS16 3490
- 2026年08月07日
- WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- Rabbit
- Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
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- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型：
Rabbit IgG
- 形态：
Lyophilized
- 保存条件：
-20°C.
- 克隆性：
Polyclonal
- 标记物：
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期：
long term storage (1 year or more) at -20°C.
- 抗原来源：
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别：
科研
- 库存：
现货
- 供应商：
上海经科化学科技有限公司
- 宿主：
Rabbit
- 应用范围：
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度：
Concentration after reconstitution: 1 µg/µL
- 抗体英文名：
Mouse IgM (µ chain), HRP conjugated
- 抗体名：
Goat anti-Mouse IgM (µ chain), HRP conjugated
- 规格：
1 mg
Goat anti-Mouse IgM (µ chain), HRP conjugated
AS16 3490| Clonality:Polyclonal| Host:Goat| Reactivity:Mouse IgM (µ chain)
Product Info
| Immunogen: | Purified Mouse IgM, µ chain |
| Host: | Goat |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified goat IgG. |
| Format: | Lyophilized |
| Quantity: | 1 mg |
| Reconstitution: | For reconstitution add 1,1 ml of sterile water,Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily |
| Storage: | Store freeze-dried powder at 2-8 °C. |
| Tested applications: | Flow cytometry (Flow cyt), Immunolocalization (IL) |
| Recommended dilution: | 1 : 20-1 : 2000 (Flow cyt), (IL) |
Reactivity
| Confirmed reactivity: | Mouse IgM (µ chain) |
Additional Information
Antibody purity is > 95% based on SDS-PAGE. Affinity purified using solid phase Mouse IgM. Antibody is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.1% (v/v) Kathon CG is added as a preservative. Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgM . Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum proteins • light chains on all mouse immunoglobulins .
Background
Goat anti-Mouse IgM (µ chain), HRP conjugated is a secondary antibody conjugated to HRP, which binds to mouse IgM (µ chain) in immunological assays.
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文献和实验Immunohistochemistry using Anti-Ganglioside Antibodies
Acetone Mouse MAb to ganglioside Mouse MAb for control experiment FITC-conjugated goat F(ab')2 fragment to mouse IgM (µ chain-specific) FITC-conjugated goat F(ab')2 fragment to mouse IgG
我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
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