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- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型:
Rabbit IgG
- 形态:
Lyophilized
- 保存条件:
-20°C.
- 克隆性:
Polyclonal
- 标记物:
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期:
long term storage (1 year or more) at -20°C.
- 抗原来源:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别:
科研
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度:
Concentration after reconstitution: 1 µg/µL
- 抗体英文名:
Sumo tag (monoclonal)
- 抗体名:
Anti-Sumo tag (monoclonal)
- 规格:
50 µg
Anti-Sumo tag (monoclonal)
AS25 5109| Clonality:Monoclonal| Host:Mouse| Reactivity:Sumo-tagged proteins
Product Info
| Immunogen: | Sumo tag protein sequence |
| Sub class: | IgG1 |
| Host: | Mouse |
| Clonality: | Monoclonal |
| Purity: | >95%, Protein G purified |
| Format: | Liquid |
| Quantity: | 50 µg |
| Storage: | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | ELISA (ELISA), Western Blot (WB) |
| Recommended dilution: | 1:500-1:5000 (WB) |
| Expected | apparent MW: | Depends upon MW of fusion partner |
Reactivity
Additional Information
Additional information (application): Antibody is present in 0.01M PBS, pH 7.4, containing 0.03% Proclin 300 as preservative and 50% Glycerol.
Background
SUMO tag monoclonal antibody is designed to specifically recognize and bind to the SUMO (Small Ubiquitin-like Modifier) tag. The SUMO tag is a peptide sequence derived from the SUMO protein, which is used in recombinant protein expression to facilitate the detection, purification, and characterization of tagged proteins.
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Anti-DYKDDDDK tag (L5) Affinity Gel
or 0.22µm filter to prepare for L5 purification. Part II. Resin Preparation 1. Thoroughly suspend the resin by gentle inversion until the bottle of Anti- DYKDDDDK-tag (L5) affinity gel is a uniform suspension of gel beads. Transfer
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说明书推荐的反应体系进行 CUT&Tag 实验;其中,pG-Tn5 转座子使用的终浓度为 0.04μM ,一抗为 H3K27me3(CST,#9733),二抗为 Goat anti Rabbit(Bioworld,#BS13271)。 A、文库峰型检测 使用 Agilent 2100 Bioanalyzer 进行文库分布检测。 图 2. 使用 TD901 构建 100 cells CUT&Tag 文库峰型图 图 3. 使用 TD901 构建 10,000 cells CUT&Tag
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein
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