
Plant Sal1 phosphatase Antibod
y 植物抗体(Anti-SAL1)相关产品推荐更多 >
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- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型:
Rabbit IgG
- 形态:
Lyophilized
- 保存条件:
-20°C.
- 克隆性:
Polyclonal
- 标记物:
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期:
long term storage (1 year or more) at -20°C.
- 抗原来源:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别:
科研
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度:
Concentration after reconstitution: 1 µg/µL
- 抗体英文名:
Sal1 phosphatase
- 抗体名:
Anti-SAL1
- 规格:
100 µl
Anti-SAL1 | Sal1 phosphatase
AS07 256| Clonality:Polyclonal| Host:Rabbit|A.thaliana, P. tremula,L. esculentum, G. max,N. tabaccum
Product Info
| Immunogen: | Recombinant SAL1, full-length protein, 353 amino acids. The cDNA of SAL1 (Q42546) was cloned into pHUE expression vector and the protein has been produced and purified according to Baker et al 2005 |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Serum |
| Format: | Lyophilized |
| Quantity: | 100 µl |
| Reconstitution: | For reconstitution add 100 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Western blot (WB) |
| Recommended dilution: | 1 : 1000 (WB) |
| Expected | apparent MW: | 37.5 | 41 kDa (Arabidopsis thaliana) |
Reactivity
| Confirmed reactivity: | Arabidopsis thaliana, Glycine max, Lycopersicum esculentum, Nicotiana tabaccum, Populus tremula |
| Predicted reactivity: | Gossypium hirsutum, Oryza sativa Species of your interest not listed? Contact us |
| Not reactive in: | Chlamydomonas reinhardii |
Additional Information
Background
The plant SAL1 (known also as FIERY1, FRY1, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 2, HOS2, MBM17.8, MBM17_8) is a 353 aa protein homologous to the HAL2 and CysQ phosphatases of yeast and Escherichia coli, respectively. The SAL1 protein expressed in E. coli shows nucleotidase and inositol phosphatase activities. SAL1 is proposed to participate in the sulfur assimilation pathway as well as in the phosphoinositide signaling pathway.
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文献和实验Assaying Levels of Plant Virus by ELISA
to confirm presence or absence of infection, it can be adapted to estimate concentration of an antigen, e.g., viral capsid protein, in plant sap. The procedure described in detail here is that for double antibody sandwich (DAS) ELISA with alkaline phosphatase
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Western blot and gel overlays(蛋白印记与凝胶覆盖)
involving a secondary antibody. The secondary antibody is raised against the primary antibody, e.g. rabbit antibody is used to make an antibody in a goat - hence "goat anti-rabbit". This secondary antibody is chemically tagged, and the tag can involve
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