
Plant Small subunit H of PSII
Antibody 植物抗体(Anti-PsbH)相关产品推荐更多 >
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- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型:
Rabbit IgG
- 形态:
Lyophilized
- 保存条件:
-20°C.
- 克隆性:
Polyclonal
- 标记物:
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期:
long term storage (1 year or more) at -20°C.
- 抗原来源:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别:
科研
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度:
Concentration after reconstitution: 1 µg/µL
- 抗体英文名:
Small subunit H of PSII
- 抗体名:
Anti-PsbH
- 规格:
100 µl
Anti-PsbH | Small subunit H of PSII
AS06 157| Clonality:Polyclonal |Host:Rabbit| Reactivity:plantsincludingA. balsamea, A.thaliana,H.vulgare,P.strobus,S.oleracea,Synechococcussp. PCC 7942,Z.mays
Product Info
| Immunogen: | KLH-conjugated synthetic peptide chosen from PsbH protein of Arabidopsis thaliana P56780, AtCg00710 |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Serum |
| Format: | Lyophilized |
| Quantity: | 100 µl |
| Reconstitution: | For reconstitution add 100 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Immunoprecipitation (IP), Western blot (WB) |
| Recommended dilution: | 1 : 5000 (WB) |
| Expected | apparent MW: | 7.7 | 4 (Arabidopsis thaliana) |
Reactivity
| Confirmed reactivity: | Abies balsamea, Arabidopsis thaliana, Hordeum vulgare, Pinus strobus, Spinacia oleracea, Synechococcus sp. PCC 7942 |
| Predicted reactivity: | Cannabis sativa, Chlamydomonas reinhardtii, Glycine max, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Picea sitchensis, Pisum sativum, Populus deltoides, Solanum lycopersicum Species of your interest not listed? Contact us |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
Additional Information
This product can be sold containing ProClin if requested Additional information (application): LMW proteins can sometimes interfere with chlorophyll, but most chlorophyll can be removed by precipitating sample in acetone before loading on a gel. Protocol: Add acetone to final concentration of 80% ice-cold acetone. Leave 10 minutes. Spin. Rresuspend pellet in solubilisation buffer and load on a gel.
Background
The PsbH protein was originally named 10- or 9-kDa phosphoprotein in higher plant chloroplasts. It is encoded by the plastome in algae and higher plants. PsbH is also present in cyanobacteria, where it exhibits 56% amino acid identity with the corresponding protein from Arabidopsis. The protein contains 63–90 amino acids, depending on the species, with molecular masses between 7.0 and 9.9 kDa. PsbH is an intrinsic membrane protein with a single transmembrane helix and its N-terminal region has been suggested to be exposed to the stromal side of the thylakoid membrane. Presence of PsbH already present in etiolated tissue can indicate that the protein may be involved in early stages of PSII assembly. Obtained biochemical data from PSII complexes isolated from spinach suggest that PsbH, together with other PSII phosphoproteins, may be required for D1 protein turnover by regulating dimeric and monomeric PSII transition through their phosphorylation and dephosphorylation.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
( 1990) Isolation of an efficient actin promoter for usein rice transformation. Plant Cell 2, 163-171. 17. Becker, D ., Brettschneider, R. and Lorz, H. (199 4 ) Fertile transgenic wheat from micro-projectile bombardment
GST-fusion protein injection into rabbits
for antibody production Depending on the antiginicity of your fusion protein, a range of 20-100 mg of fusion protein is required for first injection. NOTE: When using GST-fused to small peptide (10-20 aa) use ~250-500 mg of protein/injection.
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