
Plant DNA photolyase At4g25290
N-terminal part Antibody 植物抗体(Anti-Phly N 端)相关产品推荐更多 >
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- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide / Recombinant full-length / fragment protein expressed in E.coli
- 亚型:
Rabbit IgG
- 形态:
Lyophilized
- 保存条件:
-20°C.
- 克隆性:
Polyclonal
- 标记物:
HRP / Biotin / DyLight488 / DyLight594 / DyLight650 / FITC / TRITC (Conjugate markers vary per antibody)
- 适应物种:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii / dicots / monocots / ferns / mosses / algae (Applicable species vary per antibody)
- 保质期:
long term storage (1 year or more) at -20°C.
- 抗原来源:
Arabidopsis thaliana / Oryza sativa / Chlamydomonas reinhardtii...
- 级别:
科研
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 宿主:
Rabbit
- 应用范围:
WB / IL / IG / IP / ELISA / ChIP (Applications vary per antibody)
- 浓度:
Concentration after reconstitution: 1 µg/µL
- 抗体英文名:
DNA photolyase (At4g25290) (N-terminal part)
- 抗体名:
Anti-Phly
- 规格:
50 µg
Anti-Phly | DNA photolyase (At4g25290) (N-terminal part)
AS15 2863| Clonality:Polyclonal| Host:Rabbit| Reactivity:Arabidopsis thaliana
Product Info
| Immunogen: | KLH-conjugated peptide derived from Arabidopsis thaliana DNA photolyase, UniProt: AT4G25290, loacted in the N-terminal part of the protein |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Purity: | Immunogen affinity purified serum in PBS pH 7.4. |
| Format: | Lyophilized |
| Quantity: | 50 µg |
| Reconstitution: | For reconstitution add 50 µl of sterile water |
| Storage: | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. |
| Tested applications: | Western blot (WB) |
| Recommended dilution: | 1 : 1000 (WB) |
| Expected | apparent MW: | 78 | 90 kDa |
Reactivity
| Confirmed reactivity: | Arabidopsis thaliana |
| Not reactive in: | No confirmed exceptions from predicted reactivity are currently known |
Additional Information
Background
Protein belongs to DNA photolyases and functions in DNA repair.
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文献和实验要设立对照。该法可用于多样本、多位点甲基化的检测,样本需要量少,适于临床样本,但存在假阳性问题。 2.2.11 甲基化敏感性斑点分析(methylation sensitive dot blotassay,MS-DBA) G Clément等2005年[43]报道了一种新的方法,能够定量或半定量分析样本中的甲基化水平。这个方法的过程是:先用重亚硫酸盐处理待测DNA片段,随后以非CG区的引物行PCR扩增,将扩增产物变性后转移到尼龙膜上,用3'端DIG标记的含有2个CG(或TG)的双核苷酸探针与DNA
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
blotassay,MS-DBA) G Clément等2005年[43]报道了一种新的方法,能够定量或半定量分析样本中的甲基化水平。这个方法的过程是:先用重亚硫酸盐处理待测DNA片段,随后以非CG区的引物行PCR扩增,将扩增产物变性后转移到尼龙膜上,用3'端DIG标记的含有2个CG(或TG)的双核苷酸探针与DNA杂交,随后用带有荧光标记的抗DIG抗体与之反应。与双CG的探针获得杂交的标本含有甲基化,而与TG探针杂交的标本未被甲基化。通过比较斑点上荧光的强度测定甲基化水平。 这种方法的优点是快速、简便、易于
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