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- Biorbyt
- orb1821016
- 英国
- 2026年08月06日
- FC
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- 文献和实验
- 技术资料
- 抗体名：
Lineage Cocktail FITC Antibody 抗体
- 抗体英文名：
Lineage Cocktail FITC Antibody
- 应用范围：
FC
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1821016
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
FITC
- 保存条件：
详询
- 免疫原：
详询
- 规格：
50 T
描述: Lineage Cocktail FITC Antibody
应用说明: The Lineage Cocktail FITC is designed to stain and gate-out human T lymphocytes (CD3+), B lymphocytes (CD19+CD20+), NK cells (CD16+CD56+), non-differentiated monocytes (CD14+), neutrophils (CD16+), and eosinophils (CD14+dim). Peripheral blood dendritic cells and basophils are Lineage negative and do not stain using this reagent. Whenever applicable, the lineage negative cells can be simultaneously stained by other monoclonal antibodies conjugated to appropriate fluorochromes. For example, dendritic cells can be distinguished by positive staining with anti HLA-DR, and basophils by positive staining with anti CD203c.
防腐剂: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
研究领域: Stem Cell & Developmental Biology
Entrez 基因 ID: 35
保存条件: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
备注: For research use only.
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文献和实验. Lineage Depletion: 1. Resuspend at a density of 5x107 cells/mL in PBS/FBS plus antibody cocktail (each antibody final dilution = 1/500 v/v). 2. Incubate on ice for 30 min. Meanwhile, wash Dynabeads 2x in PBS/FBS. Resuspend the beads
Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation&
with minimal loss of surface antigen expression and antibody-antigen association. For some cell types, the presence of apoptotic cells based on reduced DNA content can also be detected. One such technique employs the addition of ethanol to cells previously
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
, and proliferation of T-lineage cells from different sources of stem cells. This article contains a series of protocols, the first of which describes the establishment, maintenance, and storage of OP9 and OP9-DL1 cells. Subsequent protocols
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