- ￥3066 - 30660
- Elabscience
- PKSR030489
- 湖北武汉
- 2026年09月11日
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- 文献和实验
- 技术资料
- 库存：
999
- 保质期：
6个月
- 供应商：
武汉伊莱瑞特生物科技股份有限公司
- 保存条件：
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
- 规格：
50 μg/1 mg
| 规格： | 50 μg | 产品价格： | ￥3066.00 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥30660.00 |
产品简称：Spike
英文名称：Recombinant SARS-CoV-2 S-trimer Protein (His Tag)
背景：The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
运输条件：This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
实验图：
Elabscience供应Recombinant SARS-CoV-2 S-trimer Protein (His Tag)，这些蛋白质中的大多数纯度≥95%，内毒素水平≤1.0EU/μg，并且已经过活性验证，助力您科学实验。
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文献和实验One-Step Purification of Recombinant Proteins with the 6xHis Tag and Ni-NTA Resin
The 6xHis/Ni-NTA system is a fast and versatile tool for the affinity purification of recombinant proteins and antigenic peptides. It is based on the high-affinity binding of six consecutive histidine residues (the 6xHis tag) to immobilized
干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密
CUT&Tag（Cleavage Under Targets and Tagmentation）是蛋白-DNA 互作的一大革新技术，它不需要使用甲醛交联以及免疫共沉淀，而是通过针对靶蛋白（如转录因子、染色质重塑蛋白）的抗体和 Protein A/G 的介导，使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头，经 PCR 扩增后即可形成用于高通量测序的文库（见图 1 ）。CUT &Tag 技术具有细胞投入量低、信噪
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
and thus fusion proteins. For example there might be sequence for a fluorescent protein such as GFP or a peptide tag such as HA upstream of MCS (and downstream of promoter) so that an insert in frame will lead to the generation of a fusion protein, an N-terminal
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