- ￥3510 - 25389
- Biorbyt
- 英国
- orb3148947
- 2026年08月06日
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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
6 months from date of receipt.
- 英文名：
Recombinant Sapporo virus VPg Protein, N-GST & C-His
- 库存：
99
- 供应商：
Biorbyt
- 规格：
50 ug/100 ug/1 mg
| 规格： | 50 ug | 产品价格： | ￥3510.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥5148.0 |
| 规格： | 1 mg | 产品价格： | ￥25389.0 |
描述: Recombinant Sapporo virus VPg Protein, N-GST & C-His is produced in E. coli and supplied in lyophilized form. The protein includes the regions of Ala940-Glu1053 and carries the N-GST & C-His tag. With a molecular weight of 41.51 kDa, it is suitable for ELISA, SDS-PAGE and WB applications. This product has a purity of greater than 90%.
形态: Lyophilized
应用: ELISA, SDS-PAGE, WB
表达区域: Ala940-Glu1053
表达系统: E. coli
分子量: 41.51 kDa
纯度: >90%
研究领域: Infectious Disease & Virology
UniProt ID: Q672I1
保存条件: -20°C
备注: For research use only.
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文献和实验Thrombin Cleavage of GST-Fusion protein
activity keeps the enzyme in aliquots at -80°C. Thrombin (MW 37kDa) ~4500units/mg. One unit cleaves theoretical 100ug protein in 16hr at 22°C in buffer. Another possibility is to use high purity enzyme from Sigma # T-6884: 3-10 U of thrombin can be used
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
expressing each construct (GST alone, GST fusion proteins) into individual 5-ml aliquots of LB broth containing appropriate antibiotic selection. Grow overnight at 37°C with shaking. Although variations of GST fusion protein expression vectors
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
REFERENCES Cox, G.N., Pratt D., McDermott M.J. and van der Slice R.W. 1999. Refolding and characterization of recombinant human soluble CTLA-4 expressed in E. coli. Protein Expr. Purif . 17 : 26-32. Frangoni J
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