- ￥7956
- Biorbyt
- orb751277
- 英国
- 2026年08月06日
- FC, IF, IHC
- Mouse
- Human
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- 文献和实验
- 技术资料
- 抗体名：
KRT14 Antibody (Biotin) 抗体
- 抗体英文名：
KRT14 Antibody (Biotin)
- 靶点：
KRT14
- 浓度：
Purified Ab conjugated to Biotin
- 应用范围：
FC, IF, IHC
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb751277
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Monoclonal
- 保存条件：
详询
- 形态：
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
- 亚型：
IgG3
- 免疫原：
详询
- 规格：
0.5 ml
描述: Mouse monoclonal antibody against KRT14 conjugated to Biotin
克隆号: LL002
别名: Anti-CK-14 antibody, anti-Dowling Meara antibody, anti-ebs3 antibody, anti-ebs4 antibody, anti-Epidermolysis Bullosa Simplex antibody, anti-Keratin Type I Cytoskeletal 14 antibody, anti-Koebner antibody, anti-NFJ antibody
应用稀释比例: Flow Cytometry (2-4ug/million cells); Immunofluorescence (2-4ug/ml); ,Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
应用说明: Positive Control: HeLa, A549, or A431 cells. Skin or squamous cell carcinoma.
免疫原: A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.
表达系统: Bioreactor
分子量: ~50kDa
防腐剂: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
纯化方法: Protein G
特异性: Expressed in the corneal epithelium (at protein level) (PubMed:26758872). Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair (PubMed:9457912). Found in keratinocytes surrounding the club hair during telogen (PubMed:9457912).
研究领域: Cell Biology, Stem Cell & Developmental Biology
UniProt ID: P02533
Entrez 基因 ID: 3861
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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