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- Biorbyt
- orb344255
- 英国
- 2026年08月06日
- ELISA, IP, WB
- Goat
- Bacteria
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- 文献和实验
- 技术资料
- 抗体名：
choA Antibody (HRP) 抗体
- 抗体英文名：
choA Antibody (HRP)
- 靶点：
choA
- 浓度：
1 mg/mL
- 应用范围：
ELISA, IP, WB
- 宿主：
Goat
- 适应物种：
Bacteria
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb344255
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
HRP
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
IgG
- 免疫原：
详询
- 规格：
100 ug
产品子类型: Primary Antibody
描述: Cholesterol Oxidase antibody (Peroxidase)
别名: goat anti-Cholesterol Oxidase Antibody HRP Conjugation, Peroxidase Conjugated goat anti-Cholesterol Oxidase Antibody, choA antibody, choD antibody, choM antibody, choS antibody, COD antibody
应用稀释比例: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
应用说明: Anti-Cholesterol Oxidase has been assayed against 1.0 ug of Cholesterol Oxidase [Microorganism] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
免疫原: Cholesterol Oxidase [Streptomyces]
防腐剂: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
纯度: Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism]. Cross reactivity against Cholesterol Oxidase from other sources is unknown.
UniProt ID: P12676
保存条件: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
备注: For research use only.
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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