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- 文献和实验
- 技术资料
- 保存条件:
2-8度
- 保质期:
1年
- 英文名:
Intein
- 库存:
999
- 供应商:
上海淳麦生物
- 规格:
/5ug/20ug/1mg
Recombinant Bacillus Circulans Intein
英文简称:
Intein
中文名称:
重组半胱氨酸蛋白G,his标签
背景资料:
Intein is a section of a protein which can remove itself and return the remaining segment with a peptide bond. In addition, Inteins hold an endonuclease domain which takes part in Intein proliferation. Actually, various genes have unrelated intein-coding segments inserted at altered positions and they were found in all thrIntein Bacillus Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 533 amino acids (3-518) and having a molecular mass of 59.4 kDa. Intein is fused to a 16 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques. domains of life (eukaryotes, bacteria, and archaea) and in viruses.
产品描述:
Intein Bacillus Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 533 amino acids (3-518) and having a molecular mass of 59.4 kDa. Intein is fused to a 16 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达
溶解建议:
提供的重组半胱氨酸蛋白G,his标签产品形式为经无菌过滤处理的无色溶液.
保存建议:
重组半胱氨酸蛋白G,his标签产品在4℃保存请2 - 4周内将使用;-20℃可长期保存.产品若长期保存,建议添加载体蛋白(0.1% HSA或BSA).避免反复冻融.
应用领域:
重组半胱氨酸蛋白G,his标签产品可广泛应用于功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词:
重组半胱氨酸蛋白G,his标签、Recombinant Bacillus Circulans Intein、Intein、重组蛋白、多肽、原核表达、His标签、CMBIO
其他简介:
CMBIO




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文献和实验Site-Specific Protein Labeling by Intein-Mediated Protein Ligation
Intein-mediated protein ligation (IPL) employs an intein to create a protein possessing a C-terminal thioester that can be ligated to a protein or peptide with an amino-terminal cysteine via a native peptide bond. Here we present a procedure
Intein-Mediated Peptide Arrays for Epitope Mapping and Kinase/Phosphatase Assays
and normalization, a new method of producing peptide arrays has been developed. This approach utilizes intein-mediated protein ligation that involves linkage of a carrier protein possessing a reactive carboxyl-terminal thioester to a peptide with an amino-terminal
Bioluminescent Imaging of MAPK Function with Intein-Mediated Reporter Gene Assay
For nondestructive analysis of chemical processes in living mammalian cells, here we show a new reporter gene assay for detecting Ras–Raf-1 interactions based on protein splicing of transcription factors with DnaE inteins. The protein
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