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- 文献和实验
- 技术资料
- 保存条件：
2-8度
- 保质期：
1年
- 英文名：
Gliadin
- 库存：
999
- 供应商：
上海淳麦生物
- 规格：
/5ug/20ug/1mg
Recombinant Wheat Gliadin
英文简称:
Gliadin
中文名称:
重组麦醇溶蛋白
背景资料:
Wheat Gliadin and related gluten components from barley， rye and possibly oats can cause an abnormal immune response called Celiac disease which is a chronic gastrointestinal disorder. Celiac disease characteristics are flattening of the jejunal mucosa and intestinal lesions of variable severity in hereditarily inclined individuals. Even though Celiac disease is not a classic autoimmune disease it is related to anti-tissue transglutaminase antibodies and gliadin antibodies tests are most recommended in scrGliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform， having a molecular mass of 18，616 Dalton （high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 47 kDa on standard SDS-PAGE）， pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes， which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.ning populations at risk for CD and other gluten-sensitive enteropathies. In the past， serologic tests for gliadin antibodies usually were not very precise and were not enough for accurate diagnosis due to missing deamidated epitopes within the authentic gliadin fraction traditionally used in diagnostic test kits. CMBIO’s deamidated Gliadin isoform matches to the deamidated neo-epitopes， which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation.
产品描述:
Gliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform， having a molecular mass of 18，616 Dalton （high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 47 kDa on standard SDS-PAGE）， pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes， which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.
来源宿主:
大肠杆菌中重组表达
溶解建议:
提供的重组麦醇溶蛋白产品形式为经无菌过滤处理的澄清溶液.
保存建议：
重组麦醇溶蛋白产品在4℃保存请2 - 4周内将使用；-20℃可长期保存.产品若长期保存，建议添加载体蛋白（0.1% HSA或BSA）.避免反复冻融.
应用领域：
重组麦醇溶蛋白产品可广泛应用于免疫检测研究、抗体开发、功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词：
重组麦醇溶蛋白、Recombinant Wheat Gliadin、Gliadin、重组蛋白、抗原、原核表达、标签蛋白、CMBIO
其他简介：
CMBIO
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文献和实验Studies of Gliadin-Specific T-Cells in Celiac Disease
of gliadin-specific T-cells derived from the small biopsy specimens. This chapter also provides guidelines for the preparation of different gliadin antigens suitable for T-cell analysis. Note that most of the T-cell assays described necessitate serological
α-氨基酸之一。 L化合物为明胶、麸蛋白（麦醇溶蛋白）、玉米醇溶蛋白、鲱精蛋白、鲑精蛋白、酪蛋白等多种蛋白的组成成分，特别是明胶中最多（约占 20％）。是唯一的醇性氨基酸，易溶于水，茚满三酮反应为黄色，由古柯叶液碱（ hygrine，属氮杂茂衍生物的生物碱）氧化所得的古柯叶液碱酸为脯氨酸的甲基衍生物，植物中存在的脯氨酸二甲内盐是二甲 -L-脯氨酸（甜菜碱）。为非必需氨基酸，在生物体内经谷氨酸被代谢。
细胞表达系统的主要优点是蛋白翻译加工后机制接近体内的天然形式，容易保留生物活性，缺点是很难高通量表达，且建立稳定细胞系的技术难度大，操作繁琐，生产成本高。 无细胞表达系统 和传统的体内表达不同，无细胞表达系统是以细胞裂解液为基础，在试管中进行的蛋白表达。无细胞蛋白表达系统是一个开放系统，不需要依赖活细胞。使用从不同物种的细胞（例如，大肠杆菌、小麦胚芽、塔氏利什曼原虫、兔网织红细胞、昆虫或人类细胞）中提取的生物分子翻译机制，在溶液中对重组蛋白质进行生产的过程。 开放性系统赋予蛋白表达的灵活性，无细胞
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