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- 文献和实验
- 技术资料
- 保存条件：
2-8度
- 保质期：
1年
- 英文名：
CDC42
- 库存：
999
- 供应商：
上海淳麦生物
- 规格：
/5ug/20ug/1mg
Recombinant Human Cell Division Cycle 42
英文简称:
CDC42
中文名称:
重组细胞分裂周期42蛋白
背景资料:
CDC42 （Cell division cycle 42 isoform 1） is a small GTPase of the Rho-subfamily that regulates signaling pathways which control various cellular functions including cell morphology， migration， endocytosis and cell cycle progression. CDC42 is a plasma membrane-associated small GTPase which cycles betwCDC42 Human Recombinant fused with a 15 amino acid T7 tag at N-terminus produced in E.Coli is a single， non-glycosylated， polypeptide chain containing 203 amino acids （1-188 a.a.） and having a molecular mass of 22.4kDa. The CDC42 is purified by proprietary chromatographic techniques.n an active GTP-bound and an inactive GDP-bound state. In the active state， CDC42 binds to a variety of e大肠杆菌中重组表达ector proteins to regulate cellular responses. CDC42 is involved in epithelial cell polarization processes. CDC42 causes the formation of thin， actin-rich surface projections called filopodia. Also， CDC42 regulates actin polymerization through its direct binding to N-WASP （Neural Wiskott-Aldrich syndrome protein）， which subsequently activates Arp2/3 complex. Loss of CDC42 function raises endocytotic uptake of apical proteins， as well as apical polarity factors such as Crumbs. The product of oncogene Dbl was described to specifically catalyze the dissociation of GDP from CDC42 protein.
产品描述:
CDC42 Human Recombinant fused with a 15 amino acid T7 tag at N-terminus produced in E.Coli is a single， non-glycosylated， polypeptide chain containing 203 amino acids （1-188 a.a.） and having a molecular mass of 22.4kDa. The CDC42 is purified by proprietary chromatographic techniques.
来源宿主:
大肠杆菌中重组表达
溶解建议:
Sterile Filtered colorless solution. The CDC42 solution contains 20mM Tris-HCl buffer （pH8.0）， 1mM DTT， 10% glycerol and 2mM EDTA.
保存建议：
CDC42 although stable 4℃ for 4 weeks， should be stored desiccated below -18℃. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
应用领域：
重组细胞分裂周期42蛋白产品可广泛应用于功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词：
重组细胞分裂周期42蛋白、Recombinant Human Cell Division Cycle 42、CDC42、重组蛋白、多肽、原核表达、标签蛋白、CMBIO
其他简介：
CMBIO
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文献和实验人β淀粉样蛋白1-42（Aβ1-42）酶联免疫分析（ELISA）
人β淀粉样蛋白1-42（Aβ1-42） 酶联免疫 分析（ELISA） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中β淀粉样蛋白1-42（Aβ1-42） 的 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 人β淀粉样蛋白1-42 （ A β 1-42 ） 水平。用纯化的 人β淀粉样蛋白1-42 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 β淀粉样蛋白1-42
Activation and Function of Small GTPases Rho, Rac, and Cdc42 During Gastrulation
activation of Rho, Rac, and Cdc42 in both mammalian cells and Xenopus embryo explants.
Identification and Functional Reconstitution of Effector Proteins for the GTPases Rac and CDC42HS
Rho-related GTPase proteins, Rat 1,2, Rho A, B, G, and CDC42Hs, constitute a distinct subfamily in the Ras super family of GTPases ( 1 ) During the last few years a large body of evidence has been accumulated that suggests that Rho
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