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- 文献和实验
- 技术资料
- 保存条件：
2-8度
- 保质期：
1年
- 英文名：
HCV NS3
- 库存：
999
- 供应商：
上海淳麦生物
- 规格：
/5ug/20ug/1mg
Recombinant Hepatitis C Virus NS3 （1450-1643）
英文简称:
HCV NS3
中文名称:
重组丙型肝炎病毒NS3（1450-1643）
背景资料:
HCV is a small 50nm， enveloped， single-stranded， positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase， the HCV has an exceptionally high mutation rate， a factor that may help it elude the host’s immune response. Hepatitis C virus is classified into six genotypes（1-6） with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes （2， 3， 5 and 6）.
产品描述:
The E. Coli derived 22kDa recombinant protein contains the HCV NS3 genotype 1b immunodominant regions， amino acids 1450-1643. The protein is fused with b-galactosidase （114 kDa） at N-terminus， pI 5.43.
来源宿主:
大肠杆菌中重组表达
溶解建议:
提供的重组丙型肝炎病毒NS3（1450-1643）产品为溶液形式.该产品已溶解在含8M 尿素和10mM B-ME的20 mM Tris-HCl缓冲液（pH 8）.
保存建议：
Five years frozen. One month in solution at room temperature.
应用领域：
重组丙型肝炎病毒NS3（1450-1643）产品可广泛应用于抗病毒研究、免疫调节研究、功能研究、蛋白互作研究、ELISA检测、Western Blot、免疫组化等生物医学研究领域。
关键词：
重组丙型肝炎病毒NS3（1450-1643）、Recombinant Hepatitis C Virus NS3 （1450-1643）、HCV NS3、重组蛋白、干扰素、原核表达、CMBIO、淳麦生物
其他简介：
CMBIO
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文献和实验Expression and Characterization of HCV NS3 Protease
The overexpression of a gene in a heterologous system is often the prelude to or the prerequisite of the elucidation and characterization of a given protein, in particular where the protein is difficult to obtain in sufficient quantity
Expression and Characterization of the HCV NS3 Helicase Domain
The hepatitis C virus (HCV) NS3 protein has two distinct biochemical domains. The N-terminal 20 kDa has serine protease activity (see Chapter 31 ) and the C-terminal 50 kDa has both nucleoside triphosphatase (NTPase) and helicase activities
Regulation of Interferon Regulatory Factor 3-Dependent Innate Immunity by the HCV NS3/4A Protease
of IRF-3 with its major protease, NS3/4A.
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