- ￥10764
- Biorbyt
- orb1248358
- 英国
- 2026年07月31日
- ELISA, FC, IF, WB
- Goat
- Human
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- 文献和实验
- 技术资料
- 抗体名：
KIT/CD117 Antibody 抗体
- 抗体英文名：
KIT/CD117 Antibody
- 靶点：
KIT / CD117
- 浓度：
500 ug/mL
- 应用范围：
ELISA, FC, IF, WB
- 宿主：
Goat
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1248358
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Liquid
- 免疫原：
详询
- 规格：
0.1 mg
产品子类型: Primary Antibody
描述: KIT/CD117 Antibody
别名: KIT, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit, CD117, PBT, SCFR, mast/stem cell growth factor receptor Kit, p145 c-kit, piebald trait protein, proto-oncogene c-Kit, proto-oncogene tyrosine-protein kinase Kit, soluble KIT varian
应用说明: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/mL. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10µg/mL. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10µg/mL.
免疫原: The immunogen for this antibody is: KRDSFICSKQEDH
分子量: Approx 110kDa
预测反应性: Bovine, Canine, Porcine
防腐剂: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
纯化方法: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
特异性: This antibody is expected to recognize both reported isoforms (NP_000213.1; NP_001087241.1).
研究领域: Immunology & Inflammation
UniProt ID: P10721
NCBI 参考序列: NP_000213.1, NP_001087241.1
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验祝贺〖雅裕睿安〗成为Signalway Antibody(SAB)独家
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制备蛋白提取物1. Prepare extracts from cultured cells or tissues with our Trident Extraction Kits.The total number of cells per ml and the cell equivalent loaded per lane of gel should be optimized specifically for each protein and antibody.2. Determine
Native Chromatin Preparation and Illumina/Solexa Library Construction
Antibody and preimmune serum for ChIP CD4+ T cell Isolation Kit II (human) (Miltenyi Biotec) Alternatively, Dynabeads Untouched Human CD4 T Cells Kit (Invitrogen) can be used (see Steps 5-21). DNA End-Repair Kit (Epicentre Biotechnologies
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